KHẢO SÁT HOẠT TÍNH KHÁNG OXY HÓA VÀ SƠ BỘ ĐỊNH LƯỢNG PHENOLIC, FLAVONOID TOÀN PHẦN TRONG DƯỢC LIỆU THÙ LÙ CẠNH (Physalis angulata)

Abstract

Background: Cutleaf groundcherry (Physalis angulata), a plant under Solanaceae family having many distinct biological activites, were studied to assess antioxidant and to analyze total phenolic, total flavonoid content on different parts (leaves, calyxes, fruits). Objectives: (1) Assess antioxidant activity using 2,2-diphenyl-1-picrylhydrazin (DPPH) and reducing power assay method; (2) Determine total phenolic and total flavonoid content using UV-Vis spectrometry. Materials and methods: Leaves, calyxes and fruits collected from Rach Gia, Kien Giang were extracted by sonication with methanol: water (70:30). The extraction was analyzed using DPPH assay, reducing power assay, Folin-Ciocalteu and aluminium chloride colormetric methods, respectively. Results: Results demonstrated that leaves extract exhibited as the highest antioxidant activity with lowest IC₅₀ (383.2 ppm) and lowest EC₅₀ (586.5 ppm), the excessive values on total phenolic and total flavonoid content, with 28.32 mg/g of acid gallic and 63.69 mg/g of quercetin equivalent per gram of dry extract. Conclusion: The study indicated that leaves, calyxes and fruits can be deemed as a good candidate for natural plant sources of antioxidants.