INVESTIGATION OF CHEMICAL CONSTITUENTS AND ANTI PROPIONIBACTERIUM ACNES ACTIVITY OF THE LICHEN USNEA UNDULATA STIRTON (PARMELIACEAE)
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Abstract
Background: Vietnamese lichens are highly diverse however the research on lichens in Vietnam has been neglected for a long time, especially in anti Propionibacterium acnes activity. Objectives: To evaluate anti P. acnes activity of Usnea undulata extracts. Isolation and structure determination of compounds in the active extracts. Materials and methods: U. undulata was collected in Lam Dong province, Vietnam. P. acnes was isolated from acne skin of patients. Using maceration and ultra sonic assisted to obtain hexane, acetone and methanol extracts. Using thin layer chromatography, column chromatography and recrystallization to isolate compounds. The antimicrobial activity against P. acnes was evaluated by disc diffusion and by broth microdilution method. Structures of isolated compounds were elucidated by spectroscopic methods including MS, NMR, and in comparison with literature data. Results: The hexane and acetone extracts showed the high anti-acnes activity with MIC 125μg/mL while no visual inhibition zone for the methanol extract at 1000μg/mL. Four compounds were isolated, including acid haemathamnolic (1), 7-hydroxyl-5-methoxy-6-methylphtalide (2), methyl orsellinate (3) và (+)–(12R)– acid usnic (4). Among them, (1) was isolated for the first time in U. undulata. Conclusions: The obtained results contributed to study on chemical constituents and anti P.acnes effect of U. undulata growing in Vietnam promising for the application of its extracts in anti-acnes cosmetics.
Keywords
acnes, lichen, Probionibacterium acnes, Usnea undulata
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