KHẢO SÁT HOẠT TÍNH CHỐNG OXY HÓA VÀ ĐỘC TẾ BÀO UNG THƯ GAN NGƯỜI HepG2 IN VITRO CỦA CÁC PHÂN ĐOẠN TỪ RỄ, THÂN XÁO TAM PHÂN [Paramignya trimera (Oliv.) Burkill]

Abstract

Background: Nowadays, Paramignya trimera (Oliv.) Burkill, Rutaceae has been used to treat some cancers; however, in Vietnam, the report on this pharmacological effect has limited. Objectives: The present work studied on in vitro antioxidant and cytotoxicity in HepG2 cells of extracts from Paramignya trimera (Oliv.) Burkill) to isolate their compounds according to guide of biological effects. Materials and methods: The stems, roots of P. trimera was hot extracted with 96% alcohol. The collected extract dissolved in 25% alcohol and liquid-liquid fractionated with nhexan, chloroform, ethyl acetate and the residual aqueous fractions. The in vitro antioxidant activity was determined by DPPH and MDA methods. The in vitro cytotoxicity in HepG2 cells was assessed by MTT test after treating HepG2 cells with extracts during 72 hours. Results: In the DPPH method, the fractionned extracts expressed their in vitro antioxidant effects in the order: chloroform, ethyl acetat extracts > n-hexan extract > aqueous extract. In the MDA method, the in vitro antioxidant activity of tested extracts exhibited in the order: n-hexan extract > chloroform extract > ethyl acetate extract > aqueous extract. The n-hexan and chloroform extracts from P. trimera roots exhibed in vitro cytotoxicity in human hepatoma HepG2 after 72h-treatment with IC₅₀ values of 36.76 µg/ml and 40.73 µg/ml, respectively. The fractioned extracts from P. trimera stems and polar extracts from P. trimera roots did not express or expressed weak cytotoxicity in HepG2 cells at the tested concentrations. Conclusion: The non-polar fractioned extracts from roots, stems of P. trimera expresssed in vitro antioxidant activity and cytotoxicity in human hepatoma HepG2; these effects were stronger than those of polar fractioned extracts.