INVESTIGATION OF THE EXTRACTION PROCESS AND PRELIMINARY PHYTOCHEMICAL ANALYSIS FROM HENNA LEAVES (LAWSONIA INERMIS L.)

Abstract

Background: Henna (Lawsonia inermis L.), also called Henna tree is a flowering plant used since antiquity to dye skin, hair, fingernails, leather, and wool for thousands of years. Henna are also used to treat injuries, bleeding, blood stasis, irregular menstrual periods. In addition, Henna are also used to treat boils, scabies and poisonous insect bites. With many uses from Henna, it is necessary to prepare solid extracts from medicinal herbs. Objective: To survey on the extraction process and preliminary phytochemical analysis from Henna leaves. Methods: Observation of the influence of extraction solvent (distilled water, 20% alcohol, 40% alcohol), the ratio of medicinal herbs and solvent (1:12; 1:14; 1:16), extraction time (30 min, 45 min, 60 min), the number of times of extraction (1 time, 2 times, 3 times) and extraction temperature (60°C, 70°C, 80°C) to the higher the extraction yield and preliminary phytochemical analysis of Henna extract. Results: It was determined that the extraction solvent was distilled water, the ratio of medicinal herbs and solvent was 1:12, the extraction time was 45 minutes, the number of times of extraction was 2 times and the extraction temperature was 60^oC. Henna leaves extract contained groups of compounds such as anthraquinones, hydrolysed triterpenoids, polyphenols and flavonoides. Conclusions: determined the extraction process and groups of compounds of Henna leaves.