SIMULTANEOUS DETERMINATION OF 6 PHENOLIC COMPOUNDS IN Physalis angulata L. FRUIT BY HPLC/DAD
Main Article Content
Abstract
Background: Currently, studies on the quantitative determination of compounds in the medicinal plant Physalis angulata L. mainly focus on the leaves or the whole plant, while studies on the fruit remain limited. Developing a procedure for quantifying phenolic compounds from Physalis angulata L. fruit is necessary to contribute to the standardization of the quality of this medicinal plant. Objectives: Simultaneous determination of 6 phenolic compounds in Physalis angulata L. fruit by HPLC/DAD and its application to fruit samples collected from several provinces in the Mekong Delta. Materials and methods: Physalis angulata L. fruits collected in Can Tho were dried to a moisture content below 13% and then used for the development and validation of phenolic compounds quantification method in accordance with AOAC guidelines. Results: Chromatographic conditions were performed using a HPLC Shimadzu LC-20A system, Phenomenex C18 column (250 x 4.6 mm; 5 µm), DAD detector at a wavelength of 254 nm, gradient program with a phase system of methanol:acetonitrile:0.2% acetic acid and 0.1% formic acid in water. The procedure met system suitability and specificity requirements; linearity range of substances from 1-150 µg/mL; The limits of detection and quantification were 0.2–1.0 µg/mL and 0.6–3.3 µg/mL, respectively; The intra-day and inter-day precision showed %RSD values below 6%; recovery at all three concentration levels was within the range of 85-110%. The validated method was successfully applied to the simultaneous determination of 6 phenolic compounds in Physalis angulata L. fruit samples collected from Can Tho, Hau Giang, and Soc Trang. Conclusions: This study presents a validated HPLC/DAD method for the simultaneous determination of six phenolic compounds in Physalis angulata L. fruit. The method was applied to the analysis of six fruit samples collected from several provinces in the Mekong Delta.
Keywords
Physalis angulata L. fruit, quantification, phenolic compounds, HPLC/DAD
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