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Issue: No. 93 (2025)
Section: Article
DOI: 10.58490/ctjump.2025i93.4284
Date Published: 25/11/2025
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Huynh, A. B., Ta, K. T., Pham, N. T. T., Pham, D. D., Do, V. A., Nguyen, T. K. N., Nguyen, T. B. H., Nguyen, V. L., & Nguyen, T. T. T. (2025). EVALUATION OF ANTIBACTERIAL ACTIVITY AND ISOLATION OF A PURE COMPOUND FROM LUDWIGIA OCTOVALVIS. Cantho Journal of Medicine and Pharmacy, 93, 86-92. https://doi.org/10.58490/ctjump.2025i93.4284

EVALUATION OF ANTIBACTERIAL ACTIVITY AND ISOLATION OF A PURE COMPOUND FROM LUDWIGIA OCTOVALVIS

An Binh Huynh1, Kien Tuong Ta1, Ngo Thanh Trung Pham1, Duc Duy Pham1, Vinh An Do1, Thi Kim Ngan Nguyen1, Thi Be Hai Nguyen1, Van Lam Nguyen1, Thi Thu Tram Nguyen1,
1 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

Background: Ludwigia octovalvis (Onagraceae) is a wild plant widely distributed in the Mekong Delta. Numerous studies have reported that this species contains a diverse range of phenolic compounds, flavonoids, and triterpenoids, exhibiting various biological activities such as antibacterial, antioxidant, and anti-inflammatory effects. However, in Vietnam, studies on the antibacterial activity and isolation of pure compounds from L. octovalvis remain limited. Objectives:  To evaluate the antibacterial activity of L. octovalvis extracts obtained by two different extraction methods, select the extract with stronger activity, and further fractionate, isolate, and elucidate the structure of pure compounds. Materials and methods: Plant materials were collected in Can Tho City from December 2023 to January 2024. Ethanol extracts were prepared using ultrasound-assisted extraction (UAE) and Soxhlet extraction (SE). Antibacterial activity was evaluated by the agar diffusion method. Column chromatography, thin-layer chromatography, and recrystallization were employed to isolate pure compounds. The structure of the isolated compound was determined using high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR) spectroscopy. Results: The ethanol extract obtained by the UAE method exhibited stronger antibacterial activity than that obtained by the SE method. The inhibition zone diameters against Staphylococcus aureus, Staphylococcus epidermidis, and Cutibacterium acnes were 13.50 mm, 15.50 mm, and 15.63 mm, respectively. From the ethyl acetate fraction, a compound was preliminarily isolated and identified as 6β-hydroxy tormentic acid. Conclusion: The ethanol extract of L. octovalvis demonstrated significant antibacterial activity against all three tested bacterial strains, and the structure of 6β-hydroxy tormentic acid was successfully isolated and structurally characterized. These findings provide scientific evidence supporting the antibacterial potential and chemical constituents of L. octovalvis collected in Can Tho City. 

Keywords

Ludwigia octovalvis, 6β-hydroxy tormentic acid, antibacterial, ultrasoundassisted extraction

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