ĐỊNH DANH MỘT SỐ CHỦNG CANDIDA CỦA PHỤ NỮ BỊ VIÊM ÂM ĐẠO BẰNG MÔI TRƯỜNG NUÔI CẤY SINH MÀU

Abstract

Background: Candida-caused vaginitis is one of common diseases in women, leading to acute and chronic recurrent lesions at the vaginal tract, and uncomfortable symptoms for patients. Objectives: 1. To determine the positive rates of some common Candida species in vaginal smear specimens of vaginitis women by using microscope and chromogenic cultured medium; 2. To evaluate the compatible level of the fresh examination under microscope and chromogenic culture vaginal smear. Materials and methods: Cross-sectional descriptive study was conducted on 160 patients examined with symptoms such as itching and abnormal vaginal discharge at the Obstetrics and Gynecology clinic - Can Tho Central General Hospital. The patient was diagnosed with vaginitis, vaginal smear was collected for fresh examination and cultured on CHROMagar medium. Results: The positive rate of Candida infection identified by microscopy method was 21.3% and chromogenic identification culture method was 33.1%. Candida albicans is the main infection agent with a positive rate detected at 66.7%, 2 times higher than Candida non-albicans (33.3%). The majority was monoinfected, accounting for 92.5%, and co-infection with different species was only 7.5%. Of monoinfection, Candida krusei accounted for 14%, Candida glabrata accounted for 14% and Candida tropicalis accounted for 5.3%. The similarity of chromogenic identification culture method and fresh examination under microscope is fair (kappa index = 0.687, p<0.05). Conclusions: Candida albicans is still the main pathogen of vaginitis, and non-Candida albicans strains are also detected in female patients with fungal vaginitis. The chromogenic identification culture method should be applied as a routine test in combination with fresh examination of vaginal smear in determining clinical Candida infection.