TÁC DỤNG BẢO VỆ CỦA QUERCETIN ĐỐI VỚI TẾ BÀO SH-SY5Y TRONG TỔN THƯƠNG THIẾU OXI–TÁI CUNG CẤP OXI

Abstract

Background: Quercetin is a flavonoid with strong antioxidant properties and has been shown to protect nerve cells in some diseases. However, the mechanism of action of Quercetin on SH-SY5Y cells has not been elucidated. Objectives: To evaluate the protective effect of Quercetin on SH-SY5Y nerve cells in hypoxia-reoxygenation injury. Materials and methods: A hypoxia incubator was used to create a hypoxia condition. SH-SY5Y cells were cultured under normal conditions (control) and hypoxia-reoxygenation conditions. The viability, reactive oxygen species, mitochondrial inner membrane cardiolipin, and extracellular BDNF protein content of SH-SY5Y cells in the experimental groups were analyzed using CCK-8, CM-H₂DCFDA, NAO, Human BDNF Elisa kits, respectively. Results: Quercetin supplementation (10÷80 µM) significantly increased the survival rate of SH-SY5Y cells under pathological conditions. In particular, Quercetin at concentrations of 10 and 20 µM showed the ability to protect cell mitochondria by inhibiting the production of reactive oxygen species, preserving cardiolipin and BDNF protein levels. Conclusions: The results obtained provide initial evidence for the effect of Quercetin in protecting mitochondria-targeted SH-SY5Y neurons against hypoxia-reoxygenation injury