XÉT NGHIỆM SÀNG LỌC NGƯỜI MANG GEN BỆNH TAN MÁU BẨM SINH (THALASSEMIA) SỬ DỤNG PHỔ HẤP THỤ PHÂN TỬ

Abstract

Background: The first step is to test the turbidity of the reagents in the OF test by molecular adsorption spectroscopy. The research aims to quantify the qualitative test from which it is possible to provide the indicators to evaluate the accuracy of the test such as the sensitivity and specificity of the measurement method. Objective: To evaluate the value of screening test for carriers of congenital hemolytic disease using molecular absorption spectroscopy. Materials and methods: 80 samples of venous blood from normal people and people carrying thalassemia gene, performed at the Department of Biochemistry - and the laboratory of Physics - Biophysics of the University of Medicine and Pharmacy. Results: The mean age of study subjects was 30±4.5. The average optical absorbance at the 416 nm peak of the negative sample is 3.45 ± 0.31, of the positive sample is 2.1 ± 0.78, the difference is statistically significant. OF test showed sensitivity and specificity of 85.2% and 80.0%, respectively. Area under the ROC curve (AUC) of Abs416=0.9; The AUC of the OF test was 0.725 (p<0.05). Conclusion: The absorption peak at 416 nm gives the best prognostic value with the area under the curve ROC = 0.90 while that of the OF test is only 0.725 when compared with the standard curve. The difference is statistically significant.