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OPTIMIZATION OF EXTRACTION B2, B3, B6, B9 VITAMINS IN FORMULA MILK POWDER
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Abstract
Background: Vitamin B plays an important role in human growth and development. Nowadays, many products help supplement vitamins such as milk formula foods, and nutritional powders on the market. Among them, formula milk powder is an important supplementary food for young children. Objective: Optimization of extraction B vitamins from formula milk powder samples. Materials and methods: B vitamins (B2, B3, B6, B9) in the formula milk powder sample were optimized for extraction and quantification using the HPLC/PDA method. Results: The optimal extraction process used 4mL of trichloroacetic acid (TCA) solvent at a concentration of 3%, ultrasonic extraction for 25 minutes, and protein precipitation repeated twice. Conclusion: The extraction method is simple, effective, repeatable and high recovery, suitable for laboratory scale to serve quantitative research on B vitamins (B2, B3, B6, B9).
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Keywords
B vitamins, formula milk powder, extraction
References
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