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OPTIMIZATION OF EXTRACTION B2, B3, B6, B9 VITAMINS IN FORMULA MILK POWDER
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Abstract
Background: Vitamin B plays an important role in human growth and development. Nowadays, many products help supplement vitamins such as milk formula foods, and nutritional powders on the market. Among them, formula milk powder is an important supplementary food for young children. Objective: Optimization of extraction B vitamins from formula milk powder samples. Materials and methods: B vitamins (B2, B3, B6, B9) in the formula milk powder sample were optimized for extraction and quantification using the HPLC/PDA method. Results: The optimal extraction process used 4mL of trichloroacetic acid (TCA) solvent at a concentration of 3%, ultrasonic extraction for 25 minutes, and protein precipitation repeated twice. Conclusion: The extraction method is simple, effective, repeatable and high recovery, suitable for laboratory scale to serve quantitative research on B vitamins (B2, B3, B6, B9).
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Keywords
B vitamins, formula milk powder, extraction
References
2. Bộ Y tế. Quy chuẩn quốc gia đối với sản phẩm dinh dưỡng công thức với mục đích ăn bổ sung cho trẻ từ 6 đến 36 tháng tuổi, QCVN 11-3/2012. 2012.
3. Bộ Y tế. Quy chuẩn quốc gia đối với sản phẩm dinh dưỡng công thức với mục đích y tế đặc biệt cho trẻ đến 12 tháng tuổi, QCVN 11-2/2012. 2012.
4. Byung Kyu Lee, Joon Hyuk Suh, Dong-Hyug Yang, Han Young Eom, Unyong Kim, Junghyun Kim, Hyeyeon Lee, and Sang Beom Han. Simultaneous Determination of B Group Vitamins inSupplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection. Bull. Korean Chemical Society. 2011. 32(8), 2648-2656. https://doi.org/10.5012/bkcs.2011.32.8.2648.
5. Soledad Albalá-Hurtado, M. Teresa Veciana-Nogués, María IzquierdoPulido, Abel MarinéFont. Determination of water-soluble vitamins in infant milk by high-performance liquid chromatography. Journal of Chromatography A. 1997. 778, 247-253, https://doi.org/10.1016/s0021-9673(97)00387-7.
6. Samanidou, V. F., & Karageorgou, E. G. An overview of the use of monoliths in sample preparation and analysis of milk. Journal of Separation Science. 2011. 34(16‐17), 2013-2025, doi:10.1002/jssc.201100101.
7. Evenson, M.L., Hinds, M.W., Bernstein, R.S., Bergdoll, M.S. Estimation of human dose of staphylococcal enterotoxin A from a large outbreaks of staphylococcal food poisoning involving chocolate milk. International Journal of Food Microbiology. 1988. 25, 311 – 316, doi:10.1016/0168-1605(88)90057-8.
8. Freed, R.C., Evenson, M.L., Reiser, R.F., Bergdoll, M.S. Enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in foods. Applied and Environmental Microbiology. 1982. 44, 1349 – 1355, doi:10.1128/aem.44.6.1349-1355.1982.
9. Meyrand, A., Atrache, V., Bavai, C., Montet, M.P., VernozyRozand, C. Evaluation of an alternative extraction procedure for enterotoxin determination in dairy products. Letters in Applied Microbiology. 1999. 28, 411 – 415, doi:10.1046/j.1365-2672.1999.00562.x.
10. Lê Thị Cúc. Xây dựng quy trình định lượng đồng thời các vitamin B1, B2, B6, B9 trong sản phẩm dinh dưỡng công thức cho trẻ em bằng HPLC. 2015. Luận văn Tốt nghiệp Dược sĩ đại học. Trường Đại học Dược Hà Nội.
11. Ren, X. N., Yin, S. A., Yang, Z. Y., Yang, X. G., Bing, S. H. A. O., Ren, Y. P., & Zhang, J. Application of UPLC-MS/MS method for analyzing B-vitamins in human milk. Biomedical and Environmental Sciences. 2015. 28(10), 738-750. doi:10.3967/bes2015.104.