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BIOASSAY - GUIDED ISOLATION OF ANTIOXIDANT CONSTITUENTS OF CURCULIGO ORCHIOIDES GAERTN. COLLECTED IN YEN BAI PROVINCE
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Abstract
Background: Curculigo orchioides was used in folk medicine as a tonic to kidney… However, chemical composition and bioactivities of this species collected in Yen Bai were not studied. Objectives: Isolation of compounds of antioxidant fraction of Curculigo orchioides. Materials and methods: Rhizomes of Curculigo orchioides collected in Yen Bai province in May 2018 and identified by Dr. Vo Van Chi. In vitro screening of extracts, fractions and isolated compounds for the antioxidant activities by the DPPH method. Pure compounds were isolated by column chromatography. Their structures were elucidated by spectrometric methods (UV, MS, NMR). Results: The ethyl acetate extract shows the most potent antioxidant activity on the DPPH method and from this fraction, 4 pure substances were isolated and elucidated as orcinol, curculigoside, curculigoside B and orcinol glucoside. These compounds exhibit weak antioxidant activities. Conclusions: From the ethyl acetate of the 96% alcohol extract, isolated and identified the structure of 4 pure substances. It’s the first time, orcinol was reported in Vietnam. These compounds exhibited weak antioxidant activities by the DPPH method.
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Keywords
Curculigo orchioides, orcinol, curculigosid, curculigosid B, orcinol glucoside
References
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