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CREATE KERATINOCYTES PLATE FROM CORD BLOOD STEM CELLS ON THE SUBSTRATE AMINONC
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Abstract
Background: Products of biological origin effective response in the treatment of burns and skin lesions loss is a matter of urgency. we proceed the study:“Create keratinocytes plate from cord blood stem cells on the substrate amnion”. Objectives: Create keratinocytes plate with multiple layers on the substrate amnion. Materials and method: The study was designed experimental description. Subjects of study were cord blood and amniotic membranes from the placenta of a full-term newborn. We collected mesenchymal stem cells from umbilical cord blood, identify and cultivate cell proliferation. Receiving and processing of the amniotic membrane into a carrier cell. Mesenchymal stem cell transplantation from umbilical cord blood to amnion. When the cell density reaches 60-80% of the area, we performed differentiation of mesenchymal stem cells into skeratinocytes. Observe under the reverse microscope to record the change in cell shape. Cell shape change reached 80%. We conducted the cascading process by airlifting method. The airliffting process was done in 7 days. Products evaluated: morphology (HE), expression of P63, CK 5/6, and TEM. Result: Successfully obtained mesenchymal stem cells from umbilical cord blood. Receive and treat the membranes successfully into cell-bearing media. Successfully create keratinocytes plate with 3-5 layers of cells. Conclusion: Successful generation of keratinocytes plate with multiple layers on the substrate amnion.
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Keywords
Mesenchymal stem cell, umbilical cord blood, keratinocyte, amnion
References
2. Mamede A. C., Carvalho M. J., Abrantes A. M., Laranjo M., Maia, C. J., Botelho M. F. Amniotic membrane: from structure and functions to clinical applications. Cell and Tissue Research. 2012. 349, 447-458. https://link.springer.com/article/10.1007/s00441-012-1424-6
3. Trần Công Toại, Nguyễn Quang Hưng, Nguyễn Hoàng Viễn Thanh. Nghiên cứu quy trình phân lập tế bào gốc máu cuống rốn ứng dụng trong nuôi cấy và biệt hóa. Tạp chí Y học thành phố Hồ Chí Minh. 2008. 12(1), 36-42.
4. Gabrielis K. Surface markers distinguishing mesenchymal stem cells from fibroblast, Acta medica lituanica. 2012. 19, 75-79. https://doi.org/10.6001/actamedica.v19i2.2313.
5. Nguyễn Minh Phương. Phân lập, nuôi cấy tế bào gốc trung mô từ mô mỡ. Luận văn thạc sĩ, Trường Đại học Y Dược Thành phố Hồ Chí Minh. 2016.
6. Chen, F. G., et al. Clonal analysis of nestin– vimentin+ multipotent fibroblasts isolated from human dermis. Journal of Cell Science. 2007. 120, 2875-2883. https://doi.org/10.1242/jcs.03478
7. Sibov T. T., Severino P., Marti L. C., Pavon L. F., Oliveira D. M., et al. Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation. Cytotechnology. 2012. 64, 511-521. https://link.springer.com/article/10.1007/s10616-012-9428-3
8. Trần Lê Bảo Hà. Nghiên cứu xác lập quy trình nuôi cấy tế bào sừng người trên màng collagen từ màng ối người hướng tới ứng dụng ghép tự thân điều trị các tổn thương mất da. Luận án tiến sĩ sinh học. Trường Đại học Khoa học Tự nhiên, Đại học Quốc Gia Thành phố Hồ Chí Minh. 2010.
9. Yang L., Shirakata Y., Shudou M., Dai X., Tokumaru S., et al. New skin-equivalent model from de-epithelialized amnion membrane. Cell Tissue Res. 2006. 326, 69-77. https://link.springer.com/article/10.1007/s00441-006-0208-2
10. Tran C.T., Huynh D.T., Gargiulo C., Nguyen P.T., Tran T.T., et al. In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture. Cell Tissue Bank. 2011. 12(2), 125-33. https://link.springer.com/article/10.1007/s10561-010-9174-8.