QUANTITATIVE DETERMINATION OF CITRONELLAL IN ESSENTIAL OIL OF Eucalyptus citriodora Hook BY GC-FID
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Abstract
Background: Essential oil from Eucalyptus citriodora Hook is commonly used in food, cosmetic and pharmaceutical industry. Quality control of essential oils, especially pharmaceutical grade, is necessary to ensure the safety and effectiveness of pharmaceutical products containing essential oils. Objectives: Essential oil was isolated by steam distillation and quantitative determination of citronellal was carried out by using a validated GC with FID detector. Materials and methods: Essential oil was obtained by steam distillation of the E. citriodora leaves collected from Long An on May, 2019. Different gas chromatographic conditions were investigated in order to obtain an optimal condition for citronellal determination. Results: Optimal parameters for essential oil extraction are: moisture content of E. citriodora leaves of 16.3%, water-leave ratio of 6:1 (mL:g), NaCl concentration of 2%, distillation time of 1 h, distillation rate of 2 mL/min. A method for quantitative determination of citronellal in essential oil was developed on Agilent 7890B gas chromatograph coupled to an FID detector; fused silica capillary HP-5 column (0.32 mm x 0.25 µm x 30 m); inlet temperature of 250oC; split ratio of 1:10; detector temperature: 280oC; inject volume: 1µl; carrier gas: nitrogen; flow rate of 3 mL/min; oven temperature program: 40oC (2 min) to 100oC at the rate of 100oC/min, hold for 1 min , to 104oC at the rate of 2oC/min, hold for 2 min, to 106oC at the rate of 2oC/min, hold for 2 min, to 110oC at the rate of 40oC/min, not hold, to 200oC at the rate 40oC/min and finally hold for 1 min; linearity range from 100 to 280 µg/mL (y = 0.6589x + 21.015, R2= 0.9982). Conclusion: The optimal condition for extracting essential oil from the leaves of Eucalyptus was developed. The validated GC-FID method enables a reliable quantitative determination of citronellal in E. citriodora essential oil.
Article Details
Keywords
Citronellal, Eucalyptus citriodora Hook, GC-FID
References
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