NGHIÊN CỨU CHIẾT XUẤT VÀ TINH CHẾ POLICOSANOL TỪ VỎ MÍA

Abstract

Background: Policosanol is a group of saturated long-chain alcohols, with chain lengths varying between 24 to 34 carbon atoms, that has been proven to be effective in reducing blood cholesterol level. Objectives: (1) Extract and purify policosanol mixture from sugarcane bark; (2) Determine policosanol composition of purified sample. Materials and methods: Sugarcane bark, a byproduct of sugarcane juice production, was collected in Can Tho city. Crude wax, obtained from sugarcane bark, was extracted and refined by solid phase extraction (SPE). Refined wax was then hydrolyzed and by fractional crystallization/ precipitation to obtain purified policosanol mixture. The purity and composition of policosanol mixture product were determined by thin layer chromatography (TLC), infrared (IR) and gas chromatography (GC-FID and GC-MS). Results: The obtained policosanol mixture from sugarcane bark was high purity product and was confirmed by TLC and GC-FID. The GC-MS analysis determined that octacosanol was the main policosanol (88,65 % in GC-MS) in sugarcane wax in Can Tho City. Conclusion: From sugarcane bark, 53,49mg high purity policosanol mixture was extracted and confirmed to be ready for pharmacological trial and pharmaceutical preparations.