XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG ĐỒNG THỜI SIBUTRAMINE VÀ PHENOLPHTHALEIN TRỘN TRÁI PHÉP TRONG THỰC PHẨM CHỨC NĂNG GIẢM CÂN BẰNG PHƯƠNG PHÁP HPLC-PDA

Abstract

Background: Sibutramine and phenolphthalein are two substances that are frequently illegally mixed in weight loss supplements. However, they were removed from the market due to the serious cardiovascular side effects of sibutramine, phenolphthalein found to be a carcinogen and also considered the cause of changes in DNA. Objectives: Determination and validation simultaneous sibutramine and phenolphthalein in weight loss supplements by HPLC and applying the procedure to analyze sibutramine and phenolphthalein in some weight loss supplement samples collected in market. Materials and Methods: sibutramine and phenolphthalein in weight loss supplements, the method was validated under AOAC guidance. Results: Chromatography condition: Restek C₁₈ column (250mm x 4,6mm; 5µm) with a gradient mode using formic acid 0,1%/acetonitrile – methanol – ammonium acetate 0,2%/methanol – water (40:60) as mobile phase. The recovery of method ranged 91.87 – 96.46% and RSD is from 1.48 – 4.11%, the LOD and LOQ were 0.33 mg/kg - 1 mg/kg, respectively. Conclusion: The results of the study showed that the proposed HPLC-PDA methods precise and accurate. The method was helpful for identification and quantification of the prohibited substances in weight loss supplements in the market.