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Date Published: 20/08/2022
Online Published: 20/08/2022
Abstract Views: 413
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DOI: https://doi.org/10.58490/ctump.2022i50.146
Issue
No. 50 (2022)
Section
Article
How to Cite
Lam, T. H. G., Vang, V. A., Ngo, T. T. H., Nguyen, L. K. K., Nguyen, T. L. L., Tran, T. P. M., Nguyen, P. D., & Vo, T. T. (2022). INVESTIGATION CHARACTERISTIC RED BLOOD CELL IN GIEMSA STAINING AND BCB STAINING OF α-THALASSEMIA MODERATE PATIENTS AT CAN THO HEMATOLOGY - BLOOD TRANSFUSION HOSPITAL IN 2021-2022. Cantho Journal of Medicine and Pharmacy, 50, 202-209. https://doi.org/10.58490/ctump.2022i50.146 (Original work published 2022)

INVESTIGATION CHARACTERISTIC RED BLOOD CELL IN GIEMSA STAINING AND BCB STAINING OF α-THALASSEMIA MODERATE PATIENTS AT CAN THO HEMATOLOGY - BLOOD TRANSFUSION HOSPITAL IN 2021-2022

Thi Huong Giang Lam1,, Van Anh Vang1, Thi Thuy Huong Ngo1, Ly Kha Ky Nguyen1, Thi Lan Linh Nguyen1, Thi Phuong Mai Tran1, Phuc Duc Nguyen1, Thanh Tri Vo1
1 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

Background: α-thalassemia trait patients are asymptomatic or have few symptoms, but they can pass α-globin genes mutated to the next generation. Moderate α-thalassemia (HbH) illustrates anemia at an average level with a variety of symptoms, causing many serious consequences, and affecting the development of the patient, family, and society. The diagnosis of α-thalassemia trait cases is necessary to limit the creation of moderate-severe forms and diagnose moderate α- thalassemia cases for timely and reasonable treatment. Peripheral blood smear with giemsa staining and BCB (Brilliant Cresyl Blue) staining slides is a vital screening technique. Objectives: To describe characteristics of erythrocytes on Giemsa and BCB. Materials and method: A cross-sectional descriptive study on 30 patients have been diagnosed α-thalassemia trait and HbH. Results: In 30 cases, 100% of red blood cells had anisocytosis (almost microcytes) and poikilocytosis, some patients had nuclear red blood cells (27%) and body Howell-Jolly (23%) on the Giemsa staining smear. The rate of reticulocyte increased with the average proportion standing at 5.37% and had HbH with an average rate of 0.23% on the BCB staining smear. Conclusion: Moderate α-thalassemia (HbH) changes red blood cells’ shape and size on the Giemsa staining, increases reticulocytes and appears HbH on BCB staining.

Article Details

Keywords

α-thalassemia, red blood cell characteristics

References

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