Article Sidebar
Views PDF: 380
IN VITRO RESEARCH OF ANTIBIOTIC RESISTANCE OF HELICOBACTER PYLORI WITH AND WITHOUT BIOFILM FORMATION (BIOFILM)
Main Article Content
Abstract
Background: Currently, the resistance of Helicobacter pylori (H. pylori) to antibiotics used in treatment regimens has reached alarming rates worldwide. Many factors are involved in drug resistance and among them is the ability of bacteria to form biofilm. Objectives: To determine in vitro of H. pylori biofilm formation ability and the antibiotic resistance rate of H. pylori with and without biofilm formation. Materials and methods: H. pylori samples were provided from the previous study in Tien Giang province to determine the ability of biofilm formation using the tissue culture plate method and to determine antibiotic resistance with amoxicillin, clarithromycin, and levofloxacin using disk-diffusion method. Results: The rate of H. pylori with and without biofilm formation was 91.5%, 8.5%. Among the samples with biofilm formation, the biofilm formation was weak, moderate, and strong are 7.0%, 20.9%, and 72.1%, respectively. The rate of H. pylori with biofilm formation resistance to amoxicillin, clarithromycin, and levofloxacin was 97.7%, 95.3%, and 2.3%, respectively. The rate of H. pylori without biofilm formation resistance to amoxicillin, clarithromycin, and levofloxacin was 75%, 100%, and 0%, respectively. The difference in antibiotic resistance rate in these two groups was not statistically significant (p>0.05). Conclusion: The rate of H. pylori with biofilm formation is high and the degree of strong biofilm formation is high. The rate of H. pylori antibiotic resistance is high. However, there was not difference in antibiotic resistance between groups with and without biofilm formation.
Article Details
Keywords
Helicobacter pylori, biofilm, antibiotic resistance, biofilm formation
References
2. Savoldi A., Carrara E., Graham D. Y., Conti M., and Tacconelli E. Prevalence of Antibiotic Resistance in Helicobacter pylori: A Systematic Review and Meta analysis in World Health Organization Regions. Gastroenterology. 2018. 155(5), 1372-1382, doi: 10.1053/j.gastro.2018.07.007.
3. Zamani M., Ebrahimtabar F., Zamani V., Miller W. H., Alizadeh-Navaei R., et al. Systematic review with meta-analysis: the worldwide prevalence of Helicobacter pylori infection. Aliment Pharmacol Ther. 2018. 47(7), 868-876, doi: 10.1111/apt.14561.
4. Vu Van Khien, Duong Minh Thang, Tran Minh Hai, Nguyen Quang Duat, Pham Hong Khanh, et al. Management of Antibiotic-Resistant Helicobacter pylori Infection: Perspectives from Vietnam. Gut and Liver. 2019. 13(5), 483-497, doi: 10.5009/gnl18137.
5. Gebreyohannes G., Nyerere A., Bii C., and Sbhatu D. B. Challenges of intervention, treatment, and antibiotic resistance of biofilm-forming microorganisms. Heliyon. 2019. 5(8), 1-7, doi: 10.1016/j.heliyon.2019.e02192.
6. Hou C., Yin F., Wang S., Zhao A., Li Y., et al. Helicobacter pylori Biofilm-Related Drug Resistance and New Developments in Its Anti-Biofilm Agents. Infection and Drug Resistance.
2022. 15, 1561-1571, doi: 10.2147/IDR.S357473.
7. Yonezawa H., Osaki T., Hojo F., and Kamiya S. Effect of Helicobacter pylori biofilm formation on susceptibility to amoxicillin, metronidazole and clarithromycin. Microbial Pathogenesis. 2019. 132 (2019), 100-108, doi: 10.1016/j.micpath.2019.04.030.
8. Fauzia K. A., Miftahussurur M., Syam A. F., Waskito L. A., Doohan D., et al. Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates. Toxins. 2020. 12(473), 1-14, doi: 10.3390/toxins12080473.
9. Trần Nguyễn Anh Huy. Nghiên cứu tình trạng đề kháng kháng sinh của vi khuẩn Helicobacter pylori ở bệnh nhân viêm, loét dạ dày-tá tràng đến khám và điều trị tại bệnh viện đa khoa trung tâm Tiền Giang. Luận văn cao học, Trường Đại hoc Y Dược Cần Thơ. 2021. 64.