INVESTIGATION OF ANTI-HELICOBACTER PYLORI ACTIVITY AND CHEMICAL CONSTITUENTS OF LUDWIGIA HYSSOPIFOLIA L.
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Abstract
Background: Ludwigia hyssopifolia was traditionally used so far for treatment of Helicobacter pylori in Mekong Delta region of Vietnam. Objectives: Preliminary evaluation of anti H. pylori activity of extracts and isolation of compounds in the active extract. Material and methods: In this study, the five extracts of L. hyssopifolia including n-hexane, chloroform, ethyl acetate, methanol and ethanol extracts were screened for anti H. pylori activity by disk diffusion method. Using column chromatography technique to isolate compounds. Their structures were elucidated by NMR spectroscopy and by comparison with literature data. Results: The results showed that all the five extracts displayed a moderate activity in which the methanol extract exhibited a stronger activity with the inhibition zone (d=17 mm) at the concentration of 0.5 mg/disk. Phytochemical study on the bioactive methanol extract led to the isolation of two known compounds as β-sitosterol (1) and 3-O-β-D-glucopyranosyl-β-sitosterol (2). Conclusion: The obtained results contribute to support experimental evidences for traditional use of L. hyssopifolia against H. pylori infection.
Keywords
antimicrobial, disk diffusion, Helicobacter pylori, Ludwigia hyssopyfolia, sterol
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