XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG ĐỒNG THỜI RUTIN VÀ LUTEOLIN TRONG THỰC PHẨM CHỨC NĂNG TRÀ HÒA TAN BẰNG PHƯƠNG PHÁP HPLC-PDA

Abstract

Background: Currently, Vietnam is producing and circulating dietary supplements extracted from Styphnolobium japonicum, Chrysanthemum indicum containing rutin and luteolin. These products have the effect of protecting blood vessel walls, increasing capillary strength, antioxidant, prevent cancer,…which are widely sold in drugstores and supermarkets. Objectives: Simultaneous determination of rutin and luteolin in soluble tea dietary supplements by HPLC-PDA. Materials and methods: Instant tea containing 6% Styphnolobium japonicum extracted and 4% Chrysanthemum indicum extracted was developed for rutin and luteolin quantification and validated analytical procedures according to AOAC guidelines. Results: The proposed method uses a Water C₁₈ column (250mm x 4.6mm x; 5µm), PDA detector (350nm) using a gradient with methanol/0.1% formic acid (45:55) as mobile phase. The method has a linear range of rutin from 0.44µg/mL442.5µg/mL; luteolin from 0.48µg/mL-191.16µg/mL. The LOD ranged at 0.1µg/mL, LOQ ranged at 0.33µg/mL. The obtained method was fully validated. The recovery of rutin ranged from 92.44%99.68% and luteolin from 91.63%-94.91%, the precision of the method ranged from 2.58% to 3.44%. The content of rutin and luteolin were respectively about 0.1mg/g and 0.04mcg/g. Conclusion: The method was proved to be suitable for the determination of rutin and luteolin in soluble tea dietary supplements.