Background: Distal humerus intercondylar fractures are challenging intra-articular injuries characterized by comminution and instability, especially in osteoporotic bone. Anatomical reduction and early mobilization are essential to restore elbow function and minimize stiffness. Among various surgical options, dual plating fixation-either perpendicular or parallel configuration-has been shown to offer reliable stability. In recent years, the integration of 3D printing and preoperative CT-based planning has improved surgical precision, reduced operative time, and optimized outcomes in complex cases. However, clinical studies evaluating the effectiveness of 3D-assisted fixation in Vietnam remain limited. Objective: This study aims to evaluate the clinical and functional outcomes of treating intercondylar humerus fractures using plate fixation assisted by preoperative 3D Computed Tomography reconstruction and 3D printing. Materials and methods: A total of 35 adult patients (≥18 years old) diagnosed with intercondylar humerus fractures (13C AO classification) were included. All patients underwent preoperative 3D Computed Tomography reconstruction and 3D model printing to assist in open reduction and internal fixation (ORIF) at Can Tho Central General Hospital and Can Tho University of Medicine and Pharmacy Hospital from October 2022 to July 2024. This prospective, descriptive study utilized convenient sampling and evaluated outcomes based on clinical examination, functional scores, and radiographic imaging. Results: The mean patient age was 42.5 ± 8.9 years, with a male-to-female ratio of 1:3. The leading causes of injury were traffic accidents (54.3%) and domestic falls (34.3%). The mean operative time was 87.2 ± 18.5 minutes, and intraoperative blood loss averaged 110 ± 22 mL. Postoperatively, 74.3% of patients achieved excellent or good functional outcomes according to Cassebaum’s rating system, and 80% had excellent to good results according to the Mayo Elbow Performance Score (MEPS). Conclusion: Preoperative 3D printing significantly enhanced surgical planning, reduced operative time and blood loss, and improved functional outcomes in treating intercondylar distal humerus fractures. This technique is a valuable adjunct in managing complex intra-articular injuries.