EVALUATION OF THE INFLUENCE OF METAL IONS ON THE SURVIVAL, ACTIVITY, AND STABILITY OF BACILLUS SPP.

Tóm tắt

Backgrounds: Bacillus renowned for their probiotic benefits, including resilience in the gastrointestinal environment and compatibility with antibiotic therapies with high-density cultivation through medium optimization has employed various statistical experimental designs but which not emphasized increasing spore yield frequently neglecting the functional properties of spores. Objectives: The purpose of the study is to determine the types of metals and metal ion concentrations that affect Bacillus subtilis M70, Bacillus clausii M31 (isolated in a laboratory in Vietnam), with the goal of enhancing its function, stability, and cell density in an optimal culture metal ion. Materials and methods: B. subtilis M70, B. clausii M31 have growth under conditions: 37°C, 200 rpm, for 18-24 hrs. Bacillus were cultured on media with varying ingredients of metal ion. A Design of Experiments (DOE) was designed in JMP Pro software (14th edition) applied. Impacts of 5 metal ions on total survival, viability efficiency, and activity of Bacillus spp. in cultures medium were examined. Results: MgSO4 and MnSO4 concentrations on Bacillus spore formation. Stability and production efficiency of Bacillus spores at CaSO4 and FeSO4 along with controlled agitation and aeration rates, maximal viability and stability were observed. High spore densities of approximately 2.06 log10 CFU/mL and significant sporulation efficiency, antibacterial ring 2.83cm and survival about 75% in pH 3 in 4 hours. Conclusion: Optimization of media constituents and culture parameters led to potential spore efficiency for Bacillus in fermentative systems, reduced nutrient requirements, increased number spores, and strengthened spore activity.