XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG PALMATIN VÀ BERBERIN TRONG BÀI THUỐC TAM HOÀNG THANG BẰNG PHƯƠNG PHÁP HPLC/PDA

Abstract

Background: Tam Hoang Thang is a classic remedy should quantify main active ingredients to improve value. Objectives: Preliminary survey conditions extract; developing and validating the process of quantifying two main ingredients in the remedy. Materials and methods: research subjects are extracts of the remedy. Reverse phase liquid chromatography method coupled with photodiode array detector is used. Results: Quantitative procedures were successfully developed with suitable chromatographic conditions: stationary phase was column Zorbax Eclipse XDB-C18, mobile phase consisting of ACN – buffer solution (contain 3.4 g potassium dihydrophosphate and 1.7 g of sodium lauryl sulfate in 1000 mL of water – ACN (1:1)) ratio 48:52; analytical wavelength 346 nm; flow rate: 0.7 mL/min; column temperature 40oC; sample injection volume: 20 µL. The procedure was validated for systematic compatibility, specificity, and linearity (palmatin (2,34 – 75 ppm): y = 19028x + 60496 với R2 = 0,9999; berberin (9,38 – 300 ppm): y = 96282x + 137909 với R2 = 0,9999); accuracy (intraday and inter-day RSD for palmatin and berberine in the range of 0.63 – 1.37); accuracy (average rate of recovery in the range 95.52 – 100.74); the LOD and LOQ of palmatin and berberin were 0.28 ppm, 0.84 ppm and 1.51 ppm, 4.56 ppm, respectively; required standards in AOAC guidelines. Conclusions: the developed and validated method can be applied to quantify simultaneously palmatin and berberin in products of Tam Hoang Thang remedy