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Date Published: 15/05/2023
Online Published: 15/05/2023
Abstract Views: 368
Views PDF: 138
Issue
No. 41 (2021)
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Article
How to Cite
Nguyen , N. L., Trinh , T. H. C., & Duong, T. L. (2023). DETECTING MYCOPLASMA PNEUMONIAE AND CHLAMYDIA PNEUMONIAE BY REAL-TIME PCR IN PATIENTS WITH COMMUNITY-ACQUIRED PNEUMONIA. Cantho Journal of Medicine and Pharmacy, 41, 107-114. https://tapchi.ctump.edu.vn/index.php/ctump/article/view/763

DETECTING MYCOPLASMA PNEUMONIAE AND CHLAMYDIA PNEUMONIAE BY REAL-TIME PCR IN PATIENTS WITH COMMUNITY-ACQUIRED PNEUMONIA

Ngoc Lam Nguyen 1,, Thi Hong Cua Trinh 2, Thi Loan Duong2
1 Tra On district Medical Center
2 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

Background: Mycoplasma pneumoniae (M. pneumoniae) and Chlamydia pneumoniae (C. pneumoniae) are two atypical bacteria causing community-acquired pneumonia (CAP) but identification is difficult by culture and serological methods. Currently, the real-time PCR technique is a molecular biology technique that helps to detect the genetic material of these two bacteria with high sensitivity and specificity. Objectives: (1) Determine the prevalence of M. pneumoniae and C. pneumoniae in patients with CAP by real-time PCR technique; (2) Find out some related factors to the prevalence of M. pneumoniae và C. pneumoniae in patients with CAP. Materials and methods: A cross-sectional descriptive study was performed on sputum samples of 157 patients diagnosed with CAP treated at the Can Tho University of Medicine and Pharmacy Hospital from July 2020 to May 2021. Results: The overall infection rate of M. pneumoniae and C. pneumoniae in sputum samples of CCP patients was 14.6% (23/157), in which M. pneumoniae accounted for 56.5% (13/23) , C. pneumoniae accounted for 8.7% (2/23) and co-infection with both bacteria was 34.8% (8/23); There was not significant  differences (p > 0.05) between the infection rates of M. pneumoniae and C. pneumoniae with the general characteristics of the patients with CAP, as well as the number of neutrophils, CRP. and lung lesions on X-ray. Conclusion: The rate of M. pneumoniae and C. pneumoniae detected by real-time PCR technique in CAP patients was 14.6% at the Can Tho University of Medicine and Pharmacy Hospital there was no relationship between the prevalence of M. pneumoniae and C. pneumoniae infection with the demographic and subclinical characteristics of CAP patients.

Article Details

Keywords

Community-acquired pneumonia (CAP), M. pneumoniae and C. pneumoniae, real-time PCR

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