NGHIÊN CỨU THÀNH PHẦN HÓA HỌC VÀ HOẠT TÍNH KHÁNG KHUẨN, KHÁNG NẤM TRONG TINH DẦU SẢ HOA HỒNG – CYMBOPOGON MARTINI (ROXB.) WILL. WATSON TRỒNG TẠI ĐẮK LẮK

Abstract

Background: Palmarosa [Cymbopogon martini (Roxb.) Will. Watson], Poaceae has a rose-like fragrance, originating from India and Turkey. Leaves of the species contain the essential oil which is a pale yellow liquid, rich in geraniol, an open-chain monoterpene. In Vietnam, there are few studies on chemical and biological activities, especially palmarosa crop in Dak Lak province. Therefore, research palmarosa essential oil planted in province is essential. Objectives: To extract, quantification of essential oils, isolation, and determination of compounds in essential oils towards anti-microbial activity. Materials and methods: palmarosa leaves collected in Dak Lak province in April, 2022. Quantification and extraction of essential oils (steam distillation method). Chromatography-coupled mass spectrometry (GC-MS), isolation (column chromatography and thin layer chromatography) and structure determination (NMR spectrometry). Test for antibacterial and antifungal activity (determination of MIC by dilution in agar). Results: Essential oil content in leaves (0.82-0.85%). The essential oil measured by GC-MS gave the ratio of compounds as geraniol (72.28%), geranyl acetate (15.91%) and linalool (3.03%). Three compounds were isolated, namely geraniol, geranyl acetate and linalool. The activity of essential oils, geraniol, geranyl acetate and linalool on Propionibacterium acnes, Aspergillus niger had MIC values of about 0.004-0.5%. Conclusions: The research of the topic contributes to the addition of the database and orientation for the research on palmarosa species.