Article Sidebar

PDF
Date Published: 12/07/2023
Online Published: 17/07/2023
Abstract Views: 396
Views PDF: 349
DOI: https://doi.org/10.58490/ctump.2023i61.553
Issue
No. 61 (2023): HỘI NGHỊ THƯỜNG NIÊN HỌC VIÊN – SINH VIÊN NGHIÊN CỨU KHOA HỌC NĂM 2023
Section
Article
How to Cite
Duong, P. T., Nguyen, T. H. Y., Nguyen, T. B. H., Quach, L. T. T., Ngo, G. T., Tran, N. P., & Cao, T. T. H. (2023). INVESTIGATION OF MIC OF COLISTIN ON BETA-LACTAM AND QUINOLONE RESISTANT ENTEROBACTERIACEAE, PSEUDOMONAS AERUGINOSA BACTERIA RESISTANT TO COLISTIN AT CAN THO UNIVERSITY OF MEDICINE AND PHARMACY HOSPITAL IN 2022-2023. Cantho Journal of Medicine and Pharmacy, 61, 253-259. https://doi.org/10.58490/ctump.2023i61.553

INVESTIGATION OF MIC OF COLISTIN ON BETA-LACTAM AND QUINOLONE RESISTANT ENTEROBACTERIACEAE, PSEUDOMONAS AERUGINOSA BACTERIA RESISTANT TO COLISTIN AT CAN THO UNIVERSITY OF MEDICINE AND PHARMACY HOSPITAL IN 2022-2023

Phuoc Trung Duong1,, Thi Hai Yen Nguyen1, Thi Be Hai Nguyen1, Le Thanh Thanh Quach1, Gia Thu Ngo1, Ngoc Phung Tran1, Thi Thu Hien Cao1
1 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

Background: Determine the minimum temperature to inhibit the growth of Colistin antibiotic to minimize the side effects of Colistin antibiotic affecting patients. Objectives: (1) To determine the rate of resistance to Beta-lactams and Quinolones of Enterobacteriaceae bacteria; rate of Colistin resistance of Pseudomonas aeruginosa strains. (2) To determine of Minimum Inhibitory Concentration (MIC) of Colistin by Broth disk elution method on Enterobacteriaceae resistant to Beta-lactams and Quinolones; Pseudomonas aeruginosa bacteria resistant to Colistin on an automated antibiogram. Materials and methods: In 208 strains of Enterobacteriaceae and P. aeruginosa bacteria causing bacterial infections, identified, and made as antibiograms according to the automatic antibiotic chart method, there were 77 resistant bacterial strains identified MIC of Colistin using Broth dis elution method. Results: Among 208 bacterial strains isolated, 77 strains (37%) showed antibiotic resistance, 53/164 (33%) Enterbacteriaceae strains showed resistance to Beta-lactam and Quinolone antibiotics; 24/44 (54.5%) bacteria Pseudomonas aeruginosa showed resistance to Colistin antibiotic. Among 77 resistant bacteria strains studied, 3 strains of bacteria with MIC of Colistin ≥4g/mL were detected: Proteus spp. showed the highest resistance with 75% (3/4), followed by Pseudomonas aeruginosa with 16.7% (4/24) and finally Klebsiella spp. with 13% (3/23) Conclusions: The rate of resistance to beta-lactam and quinolone antibiotics of Enterobacteriaceae is 32.3%; the colistin resistance rate of Pseudomonas aeruginosa strain is 54.5%. Proteus strains with MIC of Colistin ≥4g/mL accounted for the highest proportion with 75%.

Article Details

Keywords

Colistin, Pseudomonas aeruginosa, Enterobacteriaceae

References

1. Teklu D. S., Negeri A. A,, Legese M. H., Tesfaye L. B., Hiwot K. W. et al. Extended-spectrum beta-lactamase production and multi-drug resistance among Enterobacteriaceae isolated in Addis Ababa, Ethiopia. Antimicrobial Resistance & Infection Control. 2019. 8(1), 39. https://doi.org/10.1186/s13756-019-0488-4.
2. Bệnh viện Đa Khoa Khu Vực Củ Chi. Báo cáo tình hình đề kháng kháng sinh năm 2019. Bệnh viện ĐKKV Củ Chi. 2019.
3. Biagio Santella, Enrica Serretiello, Anna D. F., Folliero Veronica, Domenico Iervolino and el al. Lower Respiratory Tract Pathogens and Their Antimicrobial Susceptibility Pattern: A 5-Year Study. Antibiotics (Basel). 2021. 10 (7), 851. https://doi.org/10.3390/antibiotic10070851.
4. Bartley P. S., Nicholas Domitrovic, Vanessa T. M., Cleiton S. S., Rafael P. T. and el al.
Antibiotic Resistance in Enterobacteriaceae from Surface Waters in Urban Brazil Highlights the Risks of Poor Sanitation. Am J Trop Med Hyg. 2019. 100 (6), 1369–1377. https://doi.org/10.4269/ajtmh.18-0726.
5. Bùi Đức Long. Tình hình kháng kháng sinh tại Bệnh viện Đa khoa Hải Dương năm 2012. Y học Việt Nam. 2013. 402(1), 80-85.
6. Trần Thị Mai Hưng. Thực trạng kháng kháng sinh của một số vi khuẩn thường gặp ở cộng đồng và một số yếu tố liên quan ở Việt Nam năm 2018 - 2019. Luận án Tiến sĩ Y tế Công cộng. 2022.
7. Đoàn Ngọc Anh, Nguyễn Thanh, Phạm Hoàng Kim, Nguyễn Thị Cúc Em, Nguyễn Thị Kim Ngân. Khảo sát sự phân bố và kháng kháng sinh của các vi khuẩn thường gặp ở bệnh nhân nhiễm khuẩn tại Bệnh viện Tim mạch An Giang 10/2020 - 10/2022. Bệnh viện Tim mạch An Giang. 2022.
8. Thean Yen Tan, Lily Siew Yong Ng. Comparison of three standardized disc susceptibility testing methods for colistin. Journal of antimicrobial chemotheraphy. 2006. 58(4), 864-867. https://doi.org/10.1093/jac/dkl330.
9. Shubham Chauhan, Narinder Kaur, Adesh K. S., Jyoti Chauhan, Harit Kumar. Assessment of colistin resistance in Gram negative bacteria from clinical samples in resource-limited settings. Asian pacific journal of tropical medicine. 2022. 15(8), 367-373. https://doi.org/10.4103/19957645.351764.
10. Mohammad Qadi, S. A., Abdelraouf A. E. Colistin Resistance among Enterobacteriaceae Isolated from Clinical Samples in Gaza Strip. Canadian journal of infectious diseases and medical microbiology. 2021. 2021, 6634684. https://doi.org/10.1155/2021/6634684.