STUDY ON GENETIC, MORPHOLOGICAL, MICROSCOPIC CHARACTERISTICS, AND PRELIMINARY INVESTIGATION OF CHEMICAL COMPOSITION OF Cissus verticillata (L.) Nicolson & C.E. Jarvis, Vitaceae
Main Article Content
Abstract
Background: Cissus verticillata (L.) Nicolson & C.E. Jarvis, Vitaceae, has been extensively studied worldwide regarding its botanical characteristics, chemical composition, and pharmacognostic effects. However, there are few published studies on this species in Vietnam. Objectives: This study aims to analyze the genetic, morphological, and microscopic characteristics, as well as the preliminary chemical composition of Cissus verticillata. Materials and methods: Cissus verticillata samples were collected in Ho Chi Minh City. Genetic characteristics were evaluated via DNA extraction, amplification, sequencing, and comparison with the NCBI GenBank. Botanical methods involved documenting images of leaves, flowers, and fruits using a stereomicroscope; anatomical sections and powder of adventitious roots, stems, and leaves were conducted following Ciulei’s method. Results: Genetic analysis confirmed that the collected species was Cissus verticillata (L.) Nicolson & C.E. Jarvis, Vitaceae. The study documented morphological characteristics, anatomical features, and elements in the powder, particularly the abundance of druse and needle-shaped calcium oxalate crystals. Preliminary phytochemical screening revealed that adventitious roots contain a more diverse range of compounds compared to stems and leaves, including flavonoids, tannins, coumarins, saponins, triterpenoids, sugars, and organic acids. Conclusion: The study successfully analyzed the genetic, morphological, and microscopic characteristics, as well as the preliminary chemical composition of the species. These findings provided scientific data for the identification and further research on the chemical constituents and pharmacological effects of Cissus verticillata in Vietnam.
Keywords
Cissus verticillata, morphology, micromorphology
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References
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