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Date Published: 24/12/2022
Online Published: 24/12/2022
Abstract Views: 749
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DOI: https://doi.org/10.58490/ctump.2022i55.411
Issue
No. 55 (2022): HỘI NGHỊ KHOA HỌC SỨC KHOẺ QUỐC TẾ NĂM 2022
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Article
How to Cite
Nguyen , T. T. N., Nguyen , T. N. V., Ly, Q. T., Pham , T. M., & Le , T. T. Y. (2022). DEVELOPMENT OF EXTRACTION FOR PHENOLIC COMPOUNDS AND PRELIMINARY ASSESSMENT OF ANTIOXIDANT ACTIVITY OF RUELLIA TUBEROSA L. Cantho Journal of Medicine and Pharmacy, 55, 220-227. https://doi.org/10.58490/ctump.2022i55.411

DEVELOPMENT OF EXTRACTION FOR PHENOLIC COMPOUNDS AND PRELIMINARY ASSESSMENT OF ANTIOXIDANT ACTIVITY OF RUELLIA TUBEROSA L.

Thi Thu Ngan Nguyen 1, Thi Ngoc Van Nguyen 1,, Quoc Tuan Ly1, Thi Minh Pham 1, Thi Thanh Yen Le 1
1 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

Background: Ruellia tuberosa L. is a wild plant in the Acanthaceae family. The previous research on this plant showed antioxidant activity potential. Objectives: 1. To evaluate the in vitro antioxidant activity of extract; 2. Development of extraction procedure and chromatographic conditions for phenolic compounds in the root of Ruellia tuberosa L. extract. Materials and methods: Ruellia tuberosa L. was collected in Hau Giang province and was ultrasonically extracted with five solvents including methanol, ethanol, acetone, ethyl acetate, chloroform, and total phenolic content were determined by HPLC. Evaluated antioxidant activity by ABTS●+, DPPH, and FRAP. Results: The antioxidant activity of methanol, acetone and ethanol extracts in roots showed stronger antioxidant capacity than whole plant extracts. When methanol was selected as the extraction solvent, next investigated the ratio of methanol and methanol was. The results have shown that the methanol: water (70:30) extracted the phenolic group in the roots of Ruellia tuberosa L. extract with the highest efficiency. Conclusion: Phenolics in the root extract of the plant were higher than that of the whole plant and when extracted with methanol: water (70:30), the extraction yield of phenolics was highest.

Article Details

Keywords

Ruellia tuberosa L, phenolic compounds, anitoxidant, ABTS, DPPH, FRAP

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