RESEARCH ON TOXICITY AND IN VIVO HEPATOPROTECTIVE EFFECTS OF CAPSULE PREPARATION CONTAINING STANDARDIZED EXTRACT FROM AVICENNIA OFFICINALIS L. LEAVES ON MICE MODEL
Main Article Content
Abstract
Background: Liver diseases in recent years have been increasing rapidly due to various complex pathogenesis mechanisms and are posing a major challenge to public health. This study was conducted to evaluate the hepatoprotective effect of a capsule preparation containing Avicennia officinalis L. (AOL) leaf extract with controlled marker content on a model of acute liver injury in mice with paracetamol. Objectives: 1. To investigate the semi-chronic toxicity of the capsule to ensure safety before evaluating the hepatoprotective effect; 2. To evaluate the hepatoprotective effect of the preparation at doses of 200 mg/kg and 400 mg/kg. Materials and methods: The capsule containing the extract with marker content from Avicennia officinalis L. leaves was tested for semichronic toxicity and evaluated the hepatoprotective effect of the preparation on a model of acute liver injury in white mice with paracetamol dose of 400mg/kg. Results: The capsule containing the AOL extract did not show any signs of toxicity when used at doses of 200mg/kg/day and 400mg/kg/day, continuously for 28 days. The capsule showed significant hepatoprotective potential in the experimental model at dose of 400mg/kg. Conclusion: The capsule containing the AOL extract had a better effect in reducing AST activity and increasing antioxidant concentration than silymarin in white mice with paracetamol-induced liver injury in this experiment.
Keywords
Avicennia officinalis L., toxicity, hepatoprotective
Article Details
References
2. Đào Việt Hằng (2022), “Một số hướng nghiên cứu tiềm năng hướng tới dự phòng ung thư gan tại Việt Nam”, Hội gan mật ViệtNam.
3. Das, D. Samantaray, S.K. Sahoo, J.K. Patra, L. Samanta, H. Thatoi (2019), “Bioactivity guided isolation and structural characterization of the antidiabetic and antioxidant compound from bark extract of Avicennia officinalis L”,South African Journal of Botany,125, p.109-115.
4. F.Stickel; D. Schuppan, (2007), “Herbal medicine in the treatment of liver diseases”, Digestive and Liver Disease, 39(4), 293–304.
5. Kang, Tingguo; Dou, Deqiang; Xu, Liang (2018),”Establishment of a Quality Marker (Qmarker) System for Chinese Herbal Medicines using Burdock as an Example”, Phytomedicine
6. Mossanen, J.; Tacke, F., (2015), “Acetaminophen-induced acute liver injury in mice”, Laboratory Animals, 49(1), 30–36.
7. Nguyễn Thị Ngọc Vân, Nguyễn Huỳnh Kim Ngân, Bùi Thảo Nguyên, Lê Thị Nhân Duyên, Bùi Thị Bích Hằng (2021), “Nghiên cứu quy trình chiết xuất nhóm phenolic và đánh giá sơ bộ khả năng kháng oxy hóa in-vitro của cây Mắm ổi (Avicennia marina L.)”, Tạp chí Y Dược học Cần Thơ, 34.
8. Nhung, B. T. Q., Anh, P. T. V., Thông, N. T., Cương, L. N., & Thanh, M. P, (2023), “Tác dụng chống xơ gan của Vismisco trên mô hình gây xơ gan bằng carbon tetrachloride trên chuột nhắt trắng”, Tạp chí Nghiên cứu Y học, 56(2), 45-52.
9. Sumithra M, Janjanam VK, Kancharana VS (2011), “Influence of methanolic extract of Avicennia officinalis leaves on acute, subacute and chronic inflammatory models”, International Journal of PharmTech Research, 3, p.763-768.
10. Swagat Kumar Das, Dibyajyoti Samantaray, Archana Mahapatra, Nityasundar Pal, Rudranarayan Munda and Hrudayanath Thatoi (2018), “Pharmacological activities of leaf and bark extracts of a medicinal mangrove plant Avicennia officinalis L.”, Clinical Phytoscience, Vol 4, p.1-10.