KHẢO SÁT HOẠT TÍNH KHÁNG KHUẨN VÀ KHÁNG VIÊM IN VITRO CỦA CAO CHUẨN HÓA TỪ LÁ CÂY MẮM LƯỠI ĐỒNG (AVICENNIA OFFICINALIS L.) TẠI CÀ MAU

Abstract

Background: Avicennia officinalis L. is a tree species with strong growth capability in harsh conditions and rich in biological compounds. Pharmacological studies have shown that extracts from plant leaves and bark have antioxidant, anti-inflammatory, antibacterial, and anti-cancer properties. Objectives: To investigate the in vitro antibacterial and anti-inflammatory activities of standardized extracts from Avicennia officinalis L. leaves collected in Ca Mau. Material and methods: Antibacterial activity was assessed using the agar diffusion method and MIC determination through the agar dilution method on four bacterial strains. The in vitro antiinflammatory activity was evaluated by examining the inhibition of NO production in RAW264.7 cells. Results: The standardized leaf extract at 200 mg/mL showed antibacterial activity against Staphylococcus epidermidis (ATCC 12228), Streptococcus pneumoniae (ATCC 49619), and Streptococcus pyogenes (ATCC 19615) with inhibition zone diameters of 8.5 mm, 16 mm, and 9 mm respectively. The lowest MIC value was 2.5 mg/mL for Streptococcus pneumoniae. At 100 μg/mL, the standardized extract from Avicennia leaves reduced NO concentration comparable to the standard dexamethasone. Similarly, the percentage of NO reduction also increased gradually with the concentration of standardized extract. Conclusion: The standardized extract from Avicennia officinalis L. leaves showed antibacterial effects against 3 test strains and exhibited good antiinflammatory properties in vitro, showing potential for developing products from this plant capable of treating various inflammatory and microbial diseases.