MÔ HÌNH CHUỘT MUS MUSCULUS VIÊM GAN BỞI ETHANOL VÀ PARECETAMOL TRONG THỬ NGHIỆM THUỐC

Abstract

Background: Hepatitis is a dangerous and common disease that has been extensively studied in terms of mechanisms and treatment regimens. Various preventive and therapeutic drugs for hepatitis have been researched; however, since hepatitis can originate from different pathogenic causes, developing stable acute and chronic hepatitis mouse models is essential before assessing drug efficacy. Objective: To evaluate the effectiveness of establishing acute and chronic hepatitis models in Mus musculus mice. Materials and methods: Mus musculus mice were induced with hepatitis using ethanol (30º, 40º, 50º) and paracetamol (5%, 10%, 20% LD50 based on the AAT Bioquest system). The assessment criteria included liver morphology, AST and ALT enzyme levels, and histological changes. Results: High concentrations of ethanol and paracetamol caused mortality and structural damage to the liver. Liver enzyme levels significantly fluctuated across experimental groups. In ethanol-treated groups, cell size and liver tissue structure increased in a predictable pattern. Conclusion: Ethanol at 40º was the most effective concentration for establishing a chronic liver injury model in mice. For paracetamol-induced damage, 20% LD50 was the optimal concentration for creating an acute liver injury model.