BƯỚC ĐẦU THIẾT LẬP QUY TRÌNH THU NHẬN EXOSOME TỪ DỊCH NUÔI CẤY TẾ BÀO GỐC TRUNG MÔ TỪ MÔ MỠ HƯỚNG ĐẾN ỨNG DỤNG TRONG Y HỌC TÁI TẠO

Abstract

 Background: Exosome is opening a new direction in regenerative medicine with the ability to support, repair, and restore damaged tissue. Their potential in improving health and treating complex diseases is enormous. In the future, exosome may become a powerful and popular tool in regenerative medicine. Objectives: To initially establish a process for isolating, collecting, and identifying exosome sources to provide a standardized and safe source of exosome for research in biology and medicine. Materials and methods: Mesenchymal stem cells were isolated and expanded from human adipose tissue. The cultured cell supernatant was then used as the source material for exosome isolation. The resulting exosome product was identified based on morphology, size, and surface protein expression according to ISEV standards. The exosome product was also quantified for protein concentration using the Bradford method, and its safety was assessed for sterility and the presence of some heavy metals. Results: The exosome product after collection contained spherical exosome, with particle sizes ranging from 30 to 200 nm. By flow cytometry, the exosome particles expressed CD9, CD63, and CD81 proteins. The exosome solution had a concentration of about 45.22 µg/ml and did not contain some heavy metals surveyed, such as Lead, Cadmium, Arsenic, and Mercury. Conclusion: The exosome product solution meets ISEV international standards, ensuring safety and quality, especially for studies aimed at regenerative medicine.