NGHIÊN CỨU CHIẾT XUẤT HESPERIDIN TỪ PHỤ PHẨM VỎ CAM

Abstract

Background: Hesperidin is a very active flavonoid that is a member of the flavanone group. Nowadays, hesperidin is commonly used to treat hemorrhoids and varicose veins. Hesperidin is mostly present in the fruit peels of citrus trees, which include popular plants in Vietnam like citron, tangerines, and grapefruits. Since this raw material is now imported, studies on the extraction of hesperidin from by-products of orange peels have practical implications for proactively locating domestic source materials. Objectives: To extract and isolate hesperidin from the byproducts of orange peels. Materials and methods: Hesperidin was extracted from Cao Phong orange peels, which were collected between November 2023 and January 2024, then crushed and dried. Total flavonoids were extracted using an extraction procedure that combined a saturated Ca(OH)₂ solution with a NaOH solution, corrected the pH to 5 with an HCl solution, and allowed the mixture to crystallize for 24 hours. Use acetone and ethyl acetate solvents to separate hesperidin from total flavonoids, then decolorize with activated carbon. Product structure was determined by: IR, MS, ¹H-NMR, ¹³C-NMR  spectroscopy. Results: Total flavonoids were extracted from 200g of orange peel with the best yield of 1.28% under the conditions: Solvent-material ratio: 2/1; Extraction time 2 hours, pH 12. Hesperidin extraction with 80.82% yield from total flavonoids, R_f: 0.21 (EtOAc: MeOH: Aceton: H₂O, 10:1:1:1); Conclusion: Hesperidin was extracted from by-products of orange peel at a scale of 200 g/batch, with an overall yield of 1.03% determined from raw materials. Out of the total flavonoids, 80.82% of the hesperidin was separated.