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Date Published: 20/09/2022
Online Published: 20/09/2022
Abstract Views: 228
Views PDF: 192
DOI: https://doi.org/10.58490/ctump.2022i51.329
Issue
No. 51 (2022)
Section
Article
How to Cite
Bui, C., Nguyen, T. N. M., To, M. Q., Le, M. T., Tran, L. B. H., & Le, N. L. (2022). BIOLOGICAL CHARACTERISTICS AND BONE DEFECT COVERAGE OF NATIVE PORCINE PERICARDIUM IN VITRO. Cantho Journal of Medicine and Pharmacy, 51, 183-189. https://doi.org/10.58490/ctump.2022i51.329

BIOLOGICAL CHARACTERISTICS AND BONE DEFECT COVERAGE OF NATIVE PORCINE PERICARDIUM IN VITRO

Cuc Bui1,, Thi Ngoc My Nguyen2, Minh Quan To2, Minh Thuan Le3, Le Bao Ha Tran2, Nguyen Lam Le4
1 Dental Clinic Chau A, Ho Chi Minh City
2 The University of Science
3 Can Tho Central General Hospital
4 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

  Background: Decellularized porcine pericardium (dPP) is a suitable material for dental barrier membranes. Objectives: In this study, we aimed to determine the ability of the dPP to prevent cell invasion in vitro. Materials and methods: The dPP was gifted by Tran Le Bao Ha. In vitro biocompatibility testing was performed according to ISO 10993-5 on human gingival fibroblasts (hGF), and in vivo biocompatibility was performed according to ISO 10993-6 on Mus musculus var. Albino (n=3). The ability of dPP to prevent cell invasion was tested according to transwell migration assay: hGFs were seeded on the semi-permeable membrane covered by dPP (104 cells/well). After one day, the bottom side of SM was stained with Giemsa staining (n=3). Results: According to ISO


10993-5, hGFs remained in their elongated morphology, and cell viability was 90.33±11.33%, so dPP is not toxic to hGFs) (n=3). One month after transplant, dPP did not evoke any inflammatory response at the injury site or whole body (n=3), and few inflammatory cells presented in dPP grafts. The results of the transwell migration assay showed the hGFs couldn’t invade SM. Conclusions: dPP was not cytotoxic to fibroblast cells, did not elicit inflammatory responses in mice, and prevented cell migration in vitro. 

Article Details

Keywords

Acellular porcine pericardium, collagen membrane, cytotoxicity, in vivo biocompatibility, transwell mirgration assay

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