NGHIÊN CỨU PHƯƠNG PHÁP CHIẾT XUẤT NHÓM ACID PHENOLIC VÀ FLAVONOID TRONG LÁ CÂY MẮM ĐEN (AVICENNIA OFFICINALIS)

Abstract

Background: Avicennia officinalis is one of the many mangrove plants grown in the coastal areas of Vietnam. The groups of phenolic acid and flavonoid compounds in this plant have many important pharmacological effects such as antioxidant, antibacterial, and anti-cancer. In Vietnam, there is currently no research on the extraction method of these compounds in leaves. Objective: To develop an extraction method of phenolic acids and flavonoid compounds from the leaves of Avicennia officinalis in Thoi Binh district, Ca Mau province. Subjects and methods: The leaves of Avicennia officinalis were collected in Thoi Binh district, Ca Mau province in March 2022. The ultrasonic-assisted extraction method was selected to conduct the survey with 6 factors (extraction solvent, extraction solvent ratio, solid to liquid ratio, extraction temperature, extraction time, and times of extraction). The extraction efficiency will be evaluated through the total peak areas of phenolic acids and flavonoids by HPLC/PDA. Results: The extraction solvent selected was methanol with the ratio MeOH/water (80:20). The ratio of solid:liquid was 1:15. The ultrasonic-assisted extraction method was investigated in 20 minutes with 3 times of extraction at 45^oC. Conclusion: The optimal extraction method of phenolic acids and flavonoids compounds from Avicennia officinalis leaves achieved high extraction efficiency (>99%) and good repeatability with RSD <3%.