NGHIÊN CỨU BÀO CHẾ VÀ KHẢO SÁT HOẠT TÍNH KHÁNG KHUẨN CỦA XÀ PHÒNG LỎNG CHỨA SÀI ĐẤT BA THÙY

Abstract

Background: Sphagneticola trilobata is an immigrant species, has a fairly rich secondary metabolite composition, and is often used in folk medicine to treat skin diseases. Objectives: To prepare and evaluate the antimicrobial activity of soap-containing S. trilobata on two pathogenic bacterial strains that affect the skin of S. aureus and P. aeruginosa. Materials and methods: S. trilobata leaves collected in Da Nang, was extracted by reflux with 96% ethanol. The soap was made from a mixture of olive oil, coconut oil, and castor oil with a ratio of 6:3:1 using a mild heating method. Antimicrobial activity was investigated by disk diffusion, MIC, and IC₅₀ using microdilution methods, and thumbprint testing, while also assessing baseline standards. Results: The formulation resulted in soap containing 50 mg/mL of S. trilobata extract, which was tested against S. aureus and P. aeruginosa. The MIC values were greater than 320 µg/mL, with IC₅₀ values of 74.73 ± 2.3 µg/mL and 199.95 ± 6.3 µg/mL, respectively. Thumbprint testing indicated a higher antibacterial effect compared to the base soap, meeting standards for sensory evaluation, pH, volume uniformity, qualitative and quantitative analysis, skin irritation, heavy metal limits, preservative limits, and microbial limits according to current regulations. Conclusion: From the results obtained, data on antibacterial activity as well as applications of soap containing S. trilobata are provided.