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Date Published: 20/09/2022
Online Published: 20/09/2022
Abstract Views: 833
Views PDF: 412
DOI: https://doi.org/10.58490/ctump.2022i51.314
Issue
No. 51 (2022)
Section
Article
How to Cite
Vu, D. T., Le, T. H. M., Nguyen, A. T., Tran, D. T. T., Nguyen, T. H., Trong Nghia, & Le, T. P. (2022). HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS METHODS FOR HbA1c MEASUREMENT IN HEMOGLOBINOPATHY. Cantho Journal of Medicine and Pharmacy, 51, 82-89. https://doi.org/10.58490/ctump.2022i51.314

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS METHODS FOR HbA1c MEASUREMENT IN HEMOGLOBINOPATHY

Dinh Trung Vu1,, Thi Hoang My Le2, Anh Tu Nguyen3, Do Thao Trang Tran1, Thu Ha Nguyen4, Trong Nghia 2, Tan Phat Le2
1 Kien Giang Traditional Medicine Hospital
2 Can Tho University of Medicine and Pharmacy Hospital
3 Can Tho Hematology blood transfusion Hospital
4 Kien Giang General Hospital

Main Article Content

Abstract

  Background: In normal hemoglobin, there was no significant difference between the results obtained from both ion exchange High Pressure Liquid Chromatography (HPLC ion exchange) and Capillary Electrophoresis (CE). We do this research for comparison of HbA1c measurement methods between HPLC ion exchange and CE in hemoglobinopathy. Objectives: Comparison of the difference and compatibility of results between ion exchange high pressure liquid chromatography and capillary electrophoresis in HbA1c measurement on blood samples with hemoglobinopathy. Materials and methods: A cross-sectional descriptive study with analysis of 78 blood samples with abnormal hemoglobin at Can Tho Hematology blood transfusion Hospital and Can Tho University of Medicine and Pharmacy Hospital from April 2021 to February 2022. Results: Regarding the general characteristics of the study sample: Median of age was 20 (min 01, max 70), 43.6% blood samples were male and 56.4% were female. RBC was 5.12±1.11x1012/L, HGB was 110±27.6g/L, MCV was 70.9±9.39fL, MCH was 21.7±3.93pg. In this research, there were 23 samples of variant HbE, 13 samples of HbH and 42 samples of β-thalassemia. The different of HbA1c between HPLC and CE in total samples were -0.83±1.19(%), in group of variant HbE was -1.84±1.43(%), in group of HbH was -0.89±0.82(%), in group of β-thalassemia was -0.26±0.69(%); and incompatibility of results HbA1c assay. Conclusion: From this study, the different was -0.83±1.19(%), mean±SD of HPLC and CE was 4.25±1.25(%) and 5.08±0.56(%) respectively, p<0.05; incompatibility of results HbA1c measurement between HPLC and CE on blood samples with hemoglobinopathy.   

Article Details

Keywords

Capillary Electrophoresis, HbA1c, hemoglobinopathy, HPLC

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