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Date Published: 10/08/2024
Online Published: 10/08/2024
Abstract Views: 10
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DOI: https://doi.org/10.58490/ctump.2024i78.3102
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No. 78 (2024): HỘI NGHỊ KHOA HỌC KỶ NIỆM 10 NĂM THÀNH LẬP TRƯỜNG ĐẠI HỌC Y DƯỢC BUÔN MA THUỘT
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Nguyen, T. T. H., & Phan, H. T. (2024). DEVELOPMENT OF A QUANTIFICATION PROCESS FOR MYRICITRIN IN THE LEAF EXTRACT OF CAMEL’S FOOT TREE (Bauhinia bracteata (Benth.) Baker Fabaceae) CULTIVATED IN DAKLAK. Cantho Journal of Medicine and Pharmacy, 78, 82-88. https://doi.org/10.58490/ctump.2024i78.3102

DEVELOPMENT OF A QUANTIFICATION PROCESS FOR MYRICITRIN IN THE LEAF EXTRACT OF CAMEL’S FOOT TREE (Bauhinia bracteata (Benth.) Baker Fabaceae) CULTIVATED IN DAKLAK

Thi Thu Hanh Nguyen1,, Hoang Thu Phan1
1 Buon Ma Thuot Medicine University

Main Article Content

Abstract

Background: Bauhinia bracteata (Benth.) Baker Fabaceae) is a species with potential in the treatment of many different diseases. In Vietnam, in 2020, there was a research successfully isolated myricitrin - a flavonoid that has been proven to have many important biological activities from the ethyl acetate fraction of the leaves of the Chibi climbing plant grown in Dak Lak. Myricitrin is, however, However, the content of myricitrin in this extract has not been determined. Objectives: To develope and validating a procedure for quantifying myricitrin in the extract of the leaves of B.bracteata using the HPLC method. Materials and methods: leaves collected in Dak Lak in March 2023. Based on the structure and properties of myricitrin and reference documents, the sample preparation process and selection of chromatographic parameters of column and wavelength are provided, detection, sample injection volume, composition and ratio of mobile phase, detection wavelength to develop a procedure for quantifying myricitrin in the extract of Cinnamon leaves by HPLC method and validating the procedure according to ICH guidelines and appraisal acceptance limits regulations apply under the AOAC. Results: Quantifying myricitrin using the HPLC method with chromatography conditions including: C18 Inert Sustain chromatography column (250 x 4.6 mm; 5 µm), detection wavelength of 350 nm, injection volume sample 10 µL, mobile phase: ACN - formic acid 0.1% (20:80), flow rate 1.0 mLmin. The procedure was validated according to ICH to achieve specificity, system compatibility, repeatability (RSD < 2.0%), accuracy with recovery rate in the range of 98,30 – 100,25%, method The regression has the form y = 15208x - 442916 and the linear range of myricitrin is from 100-500 µg/mL with correlation coefficient R2 = 0.999. Conclusion: Conducting a procedure for quantifying myricitrin in leaf extract has provided important information about its content in the plant and served as a basis to help control the quality and myricitrin content of products using the leaves of the plant.

Article Details

Keywords

Bauhinia bracteata, myricitrin, quantification by HPLC

References

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