THẨM ĐỊNH PHƯƠNG PHÁP VÀ ĐỊNH LƯỢNG ALLANTOIN TRONG CỦ HOÀI SƠN (Dioscorea hamiltonii Hook.f.), HỌ CỦ NÂU (Dioscoreaceae) TRỒNG TẠI NHO QUAN, NINH BÌNH

Abstract

Background: The allantoin’s content in Dioscorea hamiltonii Hook.f., is an important criteria, but Vietnamese Pharmacopoeia V does not provide any method to quantify this substance. It is necessary to develop a method to quantify allantoin in cultivated Hoai Son tubers. Objective: To validate a method and quantify allantoin in Hoai Son tubers grown in Nho Quan, Ninh Binh. Materials and methods: Hoai Son tubers were harvested after 12 months grown in Nho Quan (Ninh Binh). A high performance liquid chromatography (HPLC) method for the quantitative analysis of allantoin has been developed.  Results: System suitability has been investigated for retention time, peak area, asymetry parameter and the theoretical number of plates. The result of this investigation showed that all values RSD < 2%. The validation procedure met the criteria for specificity, linearity and repeatability according to ICH guidelines. The contents of allantoin in Hoai Son tubers samples were determined by validated method reached 0.068% dry weight. Conclusion: The method has been validated and the allantoin content in Hoai Son tubers grown in Nho Quan, Ninh Binh was 0.068%.