KHẢO SÁT ẢNH HƯỞNG IN VITRO CỦA NANO BERBERINE LÊN MỘT SỐ ĐẶC TÍNH SINH HỌC CỦA NGUYÊN BÀO SỢI NƯỚU NGƯỜI

Abstract

Background: Berberine (BBr) is an alkaloid belonging to the isoquinoline group, which has been widely used in traditional medicine, often used to treat intestinal diseases, hepatobiliary diseases, skin diseases... Recently BBr has also been included in products.  oral care products to help promote healing.  Human gingival fibroblast (hGF) is a major component of gingival tissue, playing a key role in gingival healing. Objectives: In order to provide a scientific basis for the application of BBr in the healing of gingival-oral tissues, we carried out a research survey with the aim of evaluating the in vitro effects of Nano BBr on some biological properties (proliferation, migration) of hGF. Materials and methods: P3 generation human NBSN cells (cultured to the third pass) cultured in DMEM/F12 medium containing 10% FBS were used to evaluate the cytotoxicity of Nano Berberine on cell survival.  Nano Berberine 2% was diluted 1, 1/10, 1/102, 1/103, 1/104 to the hGF culture plates that had been incubated for 24h.  Using MTT method to test the toxicity of Nano BBr at original concentrations and diluting on hGF to determine the concentration that is not cytotoxic.  Use Nano BBr at a proven non-toxic concentration to evaluate the biological properties of hGF (proliferation, migration and collagen contraction) in cultures containing Nano BBr.  Cell culture and tests were carried out at the Laboratory of Biomedical Materials and Tissue Engineering, University of Natural Sciences, Viet Nam National University, Ho Chi Minh City. Results: With a dilution of 1/103 and 1/104 of hGF over 70%, and also with a concentration of Nano BBr 1/104, the following experiments were performed, there was proliferation and migration of hGF. Conclusions: Nano BBr 2% diluted 1/104 has a positive effect on the biological properties of hGF.