NGHIÊN CỨU HIỆU QUẢ SINH ỐNG MẦM CỦA CANDIDA ALBICAN TRONG MÔI TRƯỜNG HUYẾT THANH NGƯỜI GỘP VÀ HUYẾT THANH BÒ

Abstract

Background: Bovine serum is ideal for cell culture because it contains high levels of growth-promoting factors and is easily accessible. We conducted a study to evaluate Candida albicans' ability to produce germ tubes in a bovine serum medium in the hope of finding a suitable alternative to human serum. Objectives: To compare the agreement of germ tube test results using pooled human serum, purified bovine serum, and pure bovine serum with testing on CHROMagar medium. Materials and methods: We used vaginal fluid infected with Candida sp. in a quasiexperimental research design. Results: When compared to testing on CHROMagar medium, the germ tube test in pooled human serum medium showed a moderate level of agreement (κ = 0.59). In purified bovine serum medium, there was an acceptable level of agreement (κ = 0.37), and in pure bovine serum medium, there was a high level of agreement (κ = 0.80). Conclusion: Candida albicans can produce germ tubes in purified bovine serum medium with moderate suitability and pure bovine serum medium with even greater suitability compared to the test on CHROMagar medium. Bovine serum can be used as a substitute for pooled human serum in the germ tube test to identify Candida albicans.