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SYNTHESIZE AND EVALUATE THE ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HEXYLOXYPHENYL-1,3,4-OXADIAZOLE DERIVATIVES
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Abstract
Background: Based on the outstanding properties of natural antibacterial peptides, small molecule cationic amphipathics have been considered as potential drug development research to find novel antibacterial and antifungal agents. Based on the core structural characteristics of amphiphilic cationic antibacterial agents, the 1,3,4-oxadiazole ring that appears in many antibacterial agents was choosen to design, synthesize, and evaluate its antibacterial and antifungal activities. Objectives: To synthesize hexyloxyphenyl-1,3,4-oxadiazole derivatives and study the antibacterial and antifungal activity. Material and methods: Hexyloxyphenyl-1,3,4-oxadiazole derivatives were synthesized from the starting material 4-hydroxybenzaldehyde, then forms a 1,3,4oxadiazole ring before attaching to amino acids to obtain cationic small amphiphiles. The structures were determined by 1H NMR and 13C NMR. For testing antibacterial and antifungal activities: Microdilution method in liquid medium using broth on a 96-well plate. Result: Three hexyloxyphenyl-1,3,4-oxadiazole derivatives were synthesized. Tested activity on two bacterial strains and one fungal strain; The three synthesized substances did not show activity on the tested strains at a concentration of 128 µg/mL. Conclusion: The three hexyloxyphenyl-1,3,4-oxadiazole derivatives synthesized show non-antibacterial and non-antifungal activity on the investigated strain at a 128 µg/ml concentration. It is necessary to continue researching the synthesis of the more novel derivatives and evaluate the effects on new strains of microorganisms.
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Keywords
hexyloxyphenyl-1,3,4-oxadiazole, small molecule amphiphilic, antibacterial, antifungal
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