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Issue: No. 82 (2024)
Section: Article
DOI: 10.58490/ctump.2024i82.2989
Date Published: 25/12/2024
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Huynh, T. M. D., Le, T. M. N., Huynh, T., Doan, T. P., Nguyen, T. T., & Duong, K. V. (2024). INVESTIGATE THE EXTRACTION CONDITION OF ALLIUM ASCALONICUM L., AMARYLLIDACEAE WITH HIGH FLAVANOID CONTENT. Cantho Journal of Medicine and Pharmacy, 82, 109-115. https://doi.org/10.58490/ctump.2024i82.2989

INVESTIGATE THE EXTRACTION CONDITION OF ALLIUM ASCALONICUM L., AMARYLLIDACEAE WITH HIGH FLAVANOID CONTENT

Thi My Duyen Huynh1, Thi Minh Ngoc Le1, Tran Huynh1, Thien Phuc Doan1, Thanh Truc Nguyen1, Khanh Vy Duong1,
1 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

Background: Currently, the incidence of diabetes and dyslipidemia in our country is increasing, leading to an increased demand for pharmaceutical treatments. Shallot (Allium ascalonicum L. Amaryllidaceae) contains many flavonoid compounds that have many important pharmacological effects, notably the ability to remove free radicals and its antioxidant activity, which have potential in supporting the treatment of diabetes and dyslipidemia. However, in Viet Nam there are still few studies on investigating the extraction condition of shallot for high flavanoid content. Based on these reasons above, our team conducted an investigation on the extraction process and quantification of flavanoid in shallot. Objectives: To develop and validate the quantitative process of shallot extract using the UV-Vis spectrophotometric method and investigate the optimal conditions of shallot extraction. Materials and methods: Shallots harvested in Vinh Chau town, Soc Trang province. Quantify flavanoid using the UV-Vis spectrophotometric method based on the color reaction with AlCl3 reagent, validate the quantitative method according to ICH, AOAC guidelines and investigate the extraction conditions including ethanol concentration, medicinal herbs/solvent ratio, extraction time and temperature affecting the flavanoid content in shallot extract. Results: The process achieves specificity, precision, accuracy, regression line y= 0.0610x + 0.2864, R2 = 0.9981; 80% ethanol concentration, medical herbs/solvent ratio (1:10), extraction time (18 hours), room temperature. Conclusion: Developed and validated the quantitative process to meet the requirements according regulation and determined the optimal conditions for the optimal quercetin content of 8,34 mg QE/ g extract.

Keywords

Allium ascalonicum L., union extract, extraction process, UV-Vis

Article Details

References

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