NGHIÊN CỨU TÁC DỤNG ỔN ĐỊNH ĐƯỜNG HUYẾT TRÊN MÔ HÌNH IN-VITRO VÀ IN-VIVO CỦA CAO CHIẾT THÙ LÙ CẠNH (PHYSALIS ANGULATA L.)

Abstract

Background: Physalis angulata L. is a plant commonly used in traditional medicine, popular in tropical areas in Africa, Asia and the Americas. Physalis angulata L. is known as a herb with many biological activities such as flavonoids, phenolic acid, physalin,.. However, currently there are not many comprehensive studies in Vietnam on the pharmacological effects of stabilizing blood sugar on this plant. Objectives: 1. To investigate the ability to inhibit α-glucosidase enzyme in an in-vitro model from the extract; 2. To investigate the blood sugar stabilizing effect of the extract on an in-vivo model. Materials  and  methods: Conducting an investigation of the α-glucosidase enzyme inhibitory activity and blood sugar stabilizing effect of the extract using a glucose tolerance test in mice. Results: The extract of the leaves achieved the highest α-glucosidase inhibition efficiency of 86.55% at a concentration of 200μg/mL. In the in-vivo model, two groups of mice using the extract at two doses of 100mg/kg and 200mg/kg also demonstrated the effect of reducing glucose intolerance disorders, in which the 200mg/kg dose had a good effect on stabilizing blood sugar than. Conclusions: The leaf extract of the leaves has the effect of inhibiting the enzyme α-glucosidase, demonstrating the effect of stabilizing blood sugar in the glucose tolerance test on rats. This is considered the first step for future models to research the effect of stabilizing blood sugar in diabetes treatment.