TÌNH HÌNH SINH ENZYME β LACTAMASE PHỔ RỘNG CỦA ESCHERICHIA COLI VÀ GIÁ TRỊ CỦA CÁC PHƯƠNG PHÁP PHÁT HIỆN

Abstract

Background: Currently, Escherichia coli's extended spectrum β-lactamase production is a major concern for infectious diseases in Vietnam as well as in the world. Understanding about detection of extended spectrum β-lactamase production is very necessary. Objectives: To determine the proportion of Escherichia coli's extended spectrum β-lactamase production by the combined disk method and the phoenix M50 automatic machine; To determine the sensitivity and specificity values of the combined disk method and the phoenix M50 automatic machine for detecting extended spectrum β-lactamase-producing. Materials and method: A cross-sectional descriptive study on 155 Escherichia coli strains collected from clinical specimens of infected patients at Can Tho Central General Hospital from 7/2021-5/2022. Extended spectrum β-lactamase was detected by the combined disk test method and using the phoenix M50 automatic machine. Results: The proportion of Escherichia coli's extended spectrum β-lactamase production is 60.7% with the combined plate method and 58.9% with the Phoenix M50 automatic machine. The extended spectrum β-lactamase production assay by the combined plate method has a sensitivity and specificity of 99.1% and 98.6%, respectively. Using Phoenix M50, the sensitivity and specificity are 86.3% and 84.2%, respectively. The almost complete compatibility between the two methods of Escherichia coli's extended spectrum β-lactamase production with kappa coefficient is 0.989. Conclusion: The proportion of Escherichia coli's extended spectrum β-lactamase production by the combined plate method and the Phoenix M50 machine is quite high and the compatibility between the two methods is almost complete (kappa=0.989). Thus, the combination plate method should be recommended because of its higher efficiency.