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Date Published: 25/03/2024
Online Published: 25/03/2024
Abstract Views: 63
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DOI: https://doi.org/10.58490/ctump.2024i72.2151
Issue
No. 72 (2024)
Section
Article
How to Cite
Do , H. L., Nguyen , T. V., Le , C. H., Tran, D. H., Trinh , T. H. C., Dinh , T. H. T., & Le , C. D. (2024). APPLICATION OF GENE SEQUENCE IN DETERMINING DRUG RESISTANCE MUTANT GENOTYPES IN THE TREATMENT OF CHRONIC VIRAL HEPATITIS B. Cantho Journal of Medicine and Pharmacy, 72, 130-136. https://doi.org/10.58490/ctump.2024i72.2151

APPLICATION OF GENE SEQUENCE IN DETERMINING DRUG RESISTANCE MUTANT GENOTYPES IN THE TREATMENT OF CHRONIC VIRAL HEPATITIS B

Hoang Long Do 1,, Thanh Vu Nguyen 2, Cong Hanh Le 1, Do Hung Tran1, Thi Hong Cua Trinh 1, Thi Huong Truc Dinh 1, Chi Dung Le 1
1 Can Tho University of Medicine and Pharmacy
2 Cai Be District Medical Center

Main Article Content

Abstract

Background: Under the selective pressure of hepatitis B antiviral drugs during the treatment of chronic hepatitis B, virus strains with drug-resistant mutations will appear and spread, causing drug resistance and treatment failure. Sequencing is a molecular biology technique that helps determine the genotype of this drug resistance mutation. Objectives: To determine drug-resistant mutant genotypes for chronic hepatitis B virus treatment. Materials and method: Using the convenience sampling method, 71 chronic hepatitis B patients being treated with hepatitis B antiviral drugs such as lamivudine, adefovir, entecavir and tenofovir with positive HBsAg for over 6 months were selected for cross-sectional descriptive study performed at Can Tho University of Medicine and Pharmacy Hospital during the period from July 2022 to June 2023. Blood samples from patients with chronic hepatitis B virus that meet the standards are taken, then real-time PCR technique is carried out first and then sequencing technique later. Results: Two genotypes B and C have been identified in patients with chronic hepatitis B virus receiving antiviral treatment for hepatitis B with rates of 73.2% and 26.8%, respectively. The rate of drug resistance mutations of hepatitis B virus in patients with chronic hepatitis B virus was 7%. There were 2 lamivudine resistance mutations (V207M) and 1 adefovir resistance mutation (Q215H) detected in genotype B. In genotype C, 1 adefovir resistance mutation (Q215H) and 1 entecavir resistance mutation (S202I) were detected. Both genotypes B and C did not detect tenofovir resistance mutations. Conclusion: There are 7% drug resistance mutations of hepatitis B virus in patients with chronic hepatitis B virus. Genotype B has 2 lamivudine resistance mutations (V207M) and 1 adefovir resistance mutation (Q215H), while genotype C has 1 adefovir resistance mutation (Q215H) and 1 entecavir resistance mutation (S202I).

Article Details

Keywords

Genotype, drug resistance mutations, Hepatitis B virus

References

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