Background: Cutaneous fungal disease is a common skin disease of superficial infections, although it is not fatal, it affects aesthetics and quality of life. Objectives: (1) To determine the prevalence of cutaneous fungal infections diagnosed by microscopic examination and culture method. (2) To describe the clinical characteristics and relationships of cutaneous fungal infections. Materials and methods: This cross-sectional descriptive study was conducted in 143 patients having instructions for testing at Can Tho University of Medicine and Pharmacy Hospital Results: The prevalence of cutaneous fungal infections diagnosed by microscopic examination was 53.8% and diagnosed by culture method was 55.9 %. Compatibility level between microscopic examination and culture method shows Kappa 95.8%. The study recorded that the highest proportion of causative fungi is Candida albicans 16.1%, followed by Candida tropicalis 13.3%, and finally Trichophyton mentagrophytes 0.7%. The common clinical symptom is itching 93.75%. Common locations of damage are the face, neck 31.25% and the body 31.25%. Skin lesions include 60% papules, 50% scaly skin and the boundary of damage between healthy skin and diseased skin 30%. An association was found between cutaneous fungal infections and factors such as sweaty skin, oily skin, collective living, sharing towels and corticosteroid use (p<0.05). Conclusions: The prevalence of cutaneous fungal infections diagnosed by microscopic examination was 53.8% and diagnosed by culture method was 55.9%. The main symptom is itching 93.75%, the most common locations are the face, neck and body accounting for 31.25% respectively. It was demonstrated that sweaty skin, oily skin, collective living, sharing towels and corticosteroid use was associated with cutaneous fungal infections (p<0.05).