ĐÁNH GIÁ ĐỘC TÍNH CẤP VÀ TÁC DỤNG KIỂM SOÁT LIPID HUYẾT CỦA CAO CHIẾT ETHANOL LÁ CÂY MẬT GẤU NAM TRÊN MÔ HÌNH CHUỘT NHẮT TRẮNG TĂNG LIPID HUYẾT BẰNG TYLOXAPOL

Abstract

Background: Vernonia amygdalina - a shrub that grows predominantly in tropical Africa and is now a plant widely grown in Vietnam. It has antioxidant, anti-inflammatory, hypoglycemic, lipid-lowering effects, etc. Objectives: To evaluate the acute toxicity and protective effects of Vernonia amygdalina leaves extract on hyperlipidemia-induced by tyloxapol in Swiss albino mice. Materials and methods: Ethanolic extract of the leaves Vernonia amygdalina. The acute toxicity was conducted according to “Guidelines for preclinical and clinical trials of oriental and herbal medicines, decision number 141/QĐ-K2ĐT October 27th, 2015 of Ministry of Health”. The mice were induced hyperlipidemia by tyloxapol (400 mg/kg, intraperitoneal injection). Results: In our acute toxicity test on mice, the ethanol extract of Vernonia amygdalina did not acute oral toxicity happened at the maximum dose (D_max) (15 g/kg of body weight). In addition, mice in groups of ethanol extract of the leaves Vernonia amygdalina at a dose of 500 mg/kg, 1500 mg/kg and atorvastatin 60 mg/kg, showed a significant decrease (p < 0.05) in plasma triglyceride, but no significant decrease (p > 0.05) in plasma total cholesterol when compared with control group. Conclusion: Vernonia amygdalina leaves ethanolic extract affirms no acute toxicity. Evidence from this study proves the lipid-lowering effects of this plant at a dose of 500 mg/kg and 1500 mg/kg compared to a control group (p < 0.05), while a dose of 250 mg/kg had no effect on lowering triglycerides in mice model of hyperlipidemia with tyloxapol at a dose of 400 mg/kg.