NGHIÊN CỨU ĐỘC TÍNH CẤP, TÁC DỤNG GIẢM HO VÀ LONG ĐỜM CỦA CAO CHIẾT BÁCH BỘ TRÊN MÔ HÌNH CHUỘT

Abstract

Background: Stemona tuberosa root was often used to treat cough in folk remedies. In Dak Lak, each 2-year-old Bach Bo root can obtain 0.5 kg dried Stemona tuberosa Bo root with tuberostemonin content of 0.5%. Studies had proven that tuberostemmonin is the main component that plays an important role in the antitussive effect of Stemona tuberosa. This was the premise for the production of a cough preparation containing stemonin from Stemona tuberosa roots in Dak Lak. Objectives: To examine the safety and cough relief effect of 50% ethanol extract of Stemona tuberosa root (tuberostemonin 1.2% w/w) in experimental animals Materials and method: The acute toxicities of Stemona tuberosa extract were evaluated on mice (18 – 22 g) according to Do Trung Dam guidelines with the highest oral test dose of 25 g/kg body weight. The cough relief effect was evaluated in ammonia-induced cough model, and the expectorant effect was evaluated according to Menezes et al. with two test doses of 1.2 g/kg and 2.4 g/kg body weight. Results: Stemona tuberosa extract was considered to be non-toxic with an oral Dmax of 25 g/kg body weight. At dose of 2.4 g/kg body weight per day for 7 consecutive days, Stemona tuberosa extract could significantly reduce the number of cough episodes from 60.60 to 25.90. And Stemona tuberosa extract at a dose of 1.2 g/kg body weight twice a day had expectorant effect equivalent to N-acetyl cysteine at a dose of 5.3 mg/kg body weight. Conclusions: Stemona tuberosa extract did not cause acute toxicity at dose of 25 g/kg body weight, and had good expectorant effect and cough relief effect on the experimental model at dose of 2.4 g/kg body weight per day.