KHẢO SÁT HOẠT TÍNH KHÁNG NẤM, KHÁNG KHUẨN CỦA CAO CHIẾT RAU CÀNG CUA (PEPEROMIA PELLUCIDA) TỪ CÁC DUNG MÔI KHÁC NHAU

Abstract

Background: Peperomia pellucida is a species of plant in the family Piperaceae, which has long been used as food and medicine. Previous studies in the world have proven that Peperomia pellucida has many pharmacological effects such as antibacterial, antifungal, antioxidant, anti- inflammatory, analgesic, anti-cancer, lowering cholesterol, and heal bones but there has not been any research in Vietnam. Therefore, this topic can be considered as an important step for extensive research, analysis of pure compounds as well as evaluation of the useful pharmaceutical effects of Peperomia pellucida grown in Vietnam. Objectives: Evaluation of the antifungal and antibacterial effects of the extracts of Peperomia pellucida with different solvents. Materials and methods: Extract of Peperomia pellucida by different solvebts (water, ethanol, methanol, and ethyl acetate); Using the exhaustive method with the ratio of herbs/solvent 1:8, the extraction speed is 1 mL/min to extract the concentrated extract of Peperomia pellucida; Conduct the determination of the antifungal ability of Peperomia pellucida extracts on the fungal strains of Candida albicans and Aspergillus niger, conduct the determination of the antibacterial ability on the bacterial strains Escherichia coli and Staphylococcus aureus by agar diffusion method. Results: The diameter of the antibacterial ring against Staphylococcus aureus in ethyl acetate solution at a concentration of 200 mg/mL is 14 mm, at a concentration of 100 mg/mL is 11 mm, in addition, in the remaining trials, the antibacterial and antifungal ring diameter including the pore diameter was 8 mm (no antimicrobial activity). Conclusions: The extracts of Peperomia pellucida in water, alcohol, ethyl acetate, and methanol solvents were not able to resist Aspergillus niger, Candida albicans and Escherichia coli, Staphylococcus aureus.