RESEARCH ON THE EXTRACTION CONDITIONS OF ACETOGENINS (ACGs) IN GRAVIOLA (ANNONA MURICATA (L.) ANNONACEAE)
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Abstract
Background: Annona muricata (soursop or graviola) of Annonaceae has been reported to have a broad range of therapeutic effects since the 1940s. Graviola contains acetogenins (ACGs) that have anticancer effects and selective toxicity against various types of cancer cells even when applied at low doses. ACGs are also shown to highly inhibit an enzyme only found in cell membranes of cancerous tumors, which is the reason why the compounds are only toxic to cancer cells, not to healthy ones. The extraction of acetogenins, however, is affected by many factors such as types of solvents, the ratio of raw materials and solvents, temperature, time, and the numbers of extractions… Objectives: Studying the extraction conditions of graviola (Annona muricata (L.) Annonaceae) to determine the optimal acetogenins extraction process. Materials and methods: Testing each factor affecting the extraction process in case of remaining stable of other elements. Results: Leaves had the highest content of ACGs; Determining the optimal extraction conditions: solvent (70% of ethanol), the ratio of herbal-solvent (1:25), extraction temperature (50°C), extraction time (60 minutes), and once of extraction. Conclusion: The study determined the parts of graviola and the extraction parameters for optimal content of ACGs, which is a premise for researches on preparing products from graviola.
Keywords
graviola, acetogenins, extraction conditions
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References
2. Trần Hùng (2015), Giáo trình phương pháp nghiên cứu dược liệu, Trường Đại học Y Dược thành phố Hồ Chí Minh, tr. 2-5.
3. Alali Feras Q., Liu Xiao Xi, McLaughlin Jerry L. (1999), Annonaceous Acetogenins: Recent Progress, Journal of Natural Products, 62 (3), pp. 504-540.
4. Endrini Susi et al. (2014), Annona muricata leaves have strongest cytotoxic activitiy against breast cancer cells, Universa Medicina, 33 (3), pp. 179-184.
5. Gu Zhe-Ming, Zhao Geng-Xian (1995), Annonaceous acetogenins: Potent mitochondrial inhibitors with diverse applications, Recent Advances in Phytochemistry, 29, pp. 249-310.
6. Minari Joseph et al. (2014), Chemopreventive effect of Annona muricata on DMBAinduced cell proliferation in the breast tissues of female albino mice, Egyptian Journal of Medical Human Genetics, 15 (4), pp. 327-334.
7. Rachmani Eka Prasasti Nur, Suhesti Tuti Sri (2012), The breast of anticancer from leaf extract of Annona muricata againts cell line in T47D, International Journal of Applied Science and Technology, 2 (1), pp. 157-164.
8. Rupprecht J. Kent et al. (1990), Annonaceous Acetogenins: A review, Journal of Natural Products, 53 (2), pp. 237-278.
9. Smith Robert E., Tran Kevin and Richards Kristy M. (2014), Bioactive Annonaceous Acetogenins, Studies in Natural Products Chemistry, 41, pp. 95-115.
10. Yang Chunhua, Gundala Sushma Reddy et al. (2015), Synergistic Interactions Among Flavonoids and Acetogenins In Graviola (Annona muricata) Leaves Confer Protection Against Prostate Cancer, Carcinogenesis Advance Access, 36 (6), pp. 656-665.